The Effect Of Exercise And Glutamine Supplementation On Desmin And HGF In Rat Skeletal Muscle

Exercise-induced muscle damage (EIMI) is common in training . Recent research indicate that Z line steaming seems involve desmin which is one kind of protein of cytoskeleton. So far, hepatocyte growth factor (HGF) is the only growth factor that has the ability to stimulate activation and early division of quiescent satellite cell in skeletal muscle.During regeneration, desmin and HGF is co-expressed in muscle fibre. Glutamine is a conditionally essential amino acid, and is essential for athlete to attain muscle mass and strength. During exercise, the intramusclar concentration of glutamine is declined.Purpose: the purpose of this study is to identify the effect of glutamine supplementation on expression of desmin and HGF in exercised rats, and to explore the mechanism of improved performance through glutamine supplementation, and to explore the appropriate time for supplementing glutamine.Method: Adult SD rats were divided at random into 2-day-exercise group and 5-day-exercise group. The animals were run at a speed of 36-18m/min on a treadmill down an incline of 16?for 90 minutes. Glutamine was supplemented by gavage. The first supplementation was done immediately after the exercise, and the second was done 6-8 hours after the first one. The average amount of supplemental Gin was Ig/kg body weight ?day. In order to identify the activation of satellite cells and the development of regenerated fibers, sections of rat soleus was stained for desmin and HGF by immunohistochemical technique.Resnlt: The result indicate that glutamine seems has the ability to facilitate the expression of desmin and HGF in regenerated muscle fibre, and this happened mainly within 2 days post exercise. The cross section area of desmin-positive fiber of soleus in glutamine-supplemented rat is larger than that in placebo-supplemented rat. Since desmin may be involved in establishing and maintaining the structural integrity of muscle fibres, glutamine supplementation may be contribute to accelerate the recovery of exercise-induced muscle damage. The appropriate time of glutamine supplementation is within 5 days post exercise continuously.