1.Establishment Of Pyperinsulinemic Euglycemic Clamp Technique In Conscious Rat 2.Malnutrition In Early Phase Of Life Causes Insulin Resistance And Impaired Glucose Tolerance In Rats

Objective:To establish hyperinsulinemic euglycemic clamp technique in conscious rat, and to explore the effect of anesthesia and acute infusion of lipid on insulin sensitivity of rats. Methods:Rats were catheterized in the right jugular vein and left carotid artery after divided into two groups, and then undergoing a hyperinsulinemic- euglycemic clamp with or without infusion of lipid (Intralipid) and in unconscious and conscious state. Results:Infusion of lipid resulted in a decrease of insulin sensitivity by 30% (P<0.001 vs. control). In unconscious rats, the insulin sensitivity was decreased by 58% (P<0.001 vs. conscious). There was an antagonistic effect between infusion of lipid and anesthesia on insulin sensitivity of rats (P<0.001). Conclusion:The increase plasma free fatty acid induces insulin resistance. It would be better to carry out hyperinsulinemic-euglycemic clamp under conscious state in rats.