| Objective: Condyloma Accuminatum (CA) is a sexually transmitted disease (STDs). As the incidence rate has increased annually, it now ranks second amongst the STDs in China. CA is a hyperplasia of the epidermoma which is caused by HPV infection. HPV has an affinity for stratified pavement epithelium, and its replication occurs in completely differentiated keratin cells. The HPV route of infection is through the epithelial coloboma to inoculate the live epidermis. The probable process of CA generation is HPV infection of the basal cells, causing abnormal apoptosis, which results in abnormal increment of the cells. Podophyllotoxin is recommended by WHO as valid for CA, and 0.5% podophyllotoxin tincture is commonly used in treatment. But podophyllotoxin can only be used to treat skin damage visible to the naked eye, and serious side effects will occur if it is used over a large area. However, about 70% of genitals infected by HPV of CA are sub-clinical infections, that is, the damage is not visible to the naked eye, and can only be detected with an acetic acid test. Histological and cytological examinations can reveal significant changes. These changes bring difficulty to the clinical treatment of CA. Therefore, in order toenhance the concentration of podophyllotoxin in the skin, reduce side effects and extend the action time on the cells with pathological changes, some reform is necessary in the dose type. Based on the related literature and the appropriate medical formula sifted in advance, DPPC liposome gel and soya lecithin liposome gel were prepared.Methods: A reverse evaporation technique was adopted to make the podophyllotoxin soya lecithin liposome gel and podophyllotoxin DPPC liposome gel, and gel filtration was used to monitor their embedding ratios in different reserve conditions. Their appearance and time stability were also observed by optical and electronic microscopes. The liposome diameter distribution was examined in accordance with absorption after parallel monochromatic light irradiation of the micrones, on the basis of the laser scattering principle.Results: The form of the prepared podophyllotoxin liposome is similar to those in literature reports. High embedding ratios are shown in DPPC liposome and soya lecithin liposome, the former at 73.8%, the latter at 79.1%. The time stability is fairly good; being kept for one month below room temperature, the DPPC liposome's embedding ratio is 65.2%, after three months 58.8% and after six months 56.4%. The correspondingembedding ratios of the soya lecithin liposome are 70.3%, 60.4% and 51.3%. (The embedding ratio is slightly higher in an icebox than when below stable room temperature, but the statistics are not significant.) When inverted and observed by a biological microscope, the podophyllotoxin DPPC liposome is found to be globular, whilst the soya lecithin liposome is found to be circular with differing diameters. Observed with an electronic microscope, the DPPC liposome is multilayer and multichamber (MLV), and the soya lecithin liposome is a regular spheroidal. The maximum diameter of the DPPC liposome is 2.05 um, the minimum is 0.825um and the average is 1.45um; the corresponding diameters of soya lecithin liposome are 7.59um, 1.44 Sum and 3.78um(n=3).Conclusion: The results of the present experiment indicate that it is simple and convenient to use a reverse evaporation technique to make podophyllotoxin soya lecithin liposome gel. Since the prepared liposome is well-distributed with a high embedding ratio, it can be concluded that reverse evaporation technique is advisable. In addition, this thesis indicates that DPPC liposome is more stable than soya lecithin liposome. |
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