| Abstract Objective : To study the influence of external environment and biology of cervical cancer cell with electrochemistry in vitro and evaluate the probable mechanism of electrochemical therapy and screen reasonable clinical therapy. Methods: Four treatment dosage (5V,10C), (10V,10C), (5V,5C), (10V,5C) were used in HELA cell. MTT was used to assay inhibition rate of HELA cell in different dosage and PH at 0h,24h,48h after electrochemical therapy. To observe growth tendency of HELA cell in different dosage by counting live cell and drawing growth curve.. Automatic biochemistry analyzer and acidometer were used to estimate the changes of different ionic concentration and PH before or after electrochemical therapy and analysis the relationship of different ionic concentration and PH with growth suppression. Inverted microscope and HE were used to observe the alterant regularity of cell morphology . Flow cytometer was used to assay the influence of cell cycle. CDK1, CDK4,cyclinB1, Cydlin D1- m RNA were measured by RT-PCR. To assay relative expression of CDK, Cyclin, m RNA with semi-quantitative PCR and compare the difference. Results: (1) The inhibition rate of three segments (0h, 24h,48h) is significantly different (p<0.01). (5V,10C) and (10V,10C) group have significantly higher inhibition rate (inhibition rate >50% in every segment). The inhibition rate of (5V,5C) and (10V,5C) group is lower ( inhibition rate in 48h <50%). The subsequence of inhibition rate is (5V,10C) > (10V, 10C) > (5V,5C) > (10V, 5C). (2) When PH are in 6.54, 6.75, 7.04, 7.32 , the inhibition rate of three segments (0h, 24h,48h) has not significantly different (p>0.05), all inhibition rate is lower than 50%. When PH are in 10.07,9.95,9.42,9.53 , the inhibition rate has significantly different (p<0.05-0.01). The inhibition is increasing when the PH is heightening. PH 10.07 has obvious inhibition, all inhibition rate is higher than 50%., The inhibition rate is significantly higher in alkali culture medium than in acid culture medium (p<=0.01). (3) The inhibition rate with electrochemical therapy is significantly higher than with acid and alkali .The subsequence of inhibition rate is electrochemical therapy > alkali> acid. (4)Controlling the influence of time, the cell number of (5V,10C) (10V, 10C) , (10V,5C) (5V,5C) and blank group has significantly different (p<0.01). From cell growth curve, the cell number is increasing with time in blank group. The cell number of (5V,5C) (10V,5C) reduce at beginning. After three days, the cell number is increasing gradually, and then has coincident growth tendency with blank group. The cell number of (5V,10C) (10V,10C) group is reducing gradually and has significantly different comparing with other 3 group. (5) Automatic biochemistry analyzer displays that, after electrochemical therapy, the concentration of K+,Ca2+,Mg2+ at cathode of (5V,10C) is significantly higher than contrast group (p<0.01) and that the concentration of K+,Na2+ at cathode of (10V,10C) is significantly higher than contrast group (p<0.01). But, the concentration of Cl - at anode in every treatment group is significantly higher than contrast group (p<0.05-0.01). Comparing the change of different ionic concentration in treatment group, the increase range of K+ at cathode of (5V,10C) and (10V,10C) group is significantly higher than the other treatment group (P<0.01). The increase range of Ca2+ is significantly higher at cathode of (5V,10C) than the other three group (p<0.01) and the decrease range of Mg2+ at anode is significantly higher than the other three group (p<=0.05). (6) Comparing electrochemical therapy group with blank group, PH increases obviously to alkali at cathode. PH decreases significantly to acidity at anode (p<0.05-0.01). After electrochemical therapy, PH at cathode in every group has no significantly different (p>0.05). But PH at anode changes significantly (F=13.37,P=0.02). PH has the biggest change in (5V,10C) group and it's descent range is significantly higher than (5V,5C) and (10V,5C), p<=0.01. (7) Af...
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