The Study Of The Mechanism Of The Electrochemical Therapy Of Human Breast Cancer MCF-7Cell Line

Objectives This experiment through detecting cultured breast cancer cells such as thechange of PH value,the apoptosis and growth of breast cancer cell,cell cycle changed,thechanges of detecting intracellular calcium,and the expression of c-myc and cyclin E changedbefore and after the electrochemical treatment,so that we could further explore the mechanismof electrochemical therapy induced human breast cancer in vitro about cell cycle phase and partof the mechanism of its inhibition about cell growth and induced.apoptosis.Methods Culctured MCF-7cells,6hours and24hours ’after treatment, through acidestimation,MTT method,GENMED Calcium Colorimetric Assay Kit,flow cytometry, confocallaser and immunohistochemical method respectively deteced cell culture fluid PH value, theinhibitory rate,intracellular Ca²⁺concentration,cell cycle,observing the apoptosis and necrosisand the expression of c-myc and cyclin E changed.Results Compared with the control group,after The electrochemical therapy,the cathodePH value is significantly higher,alkaline; anode PH value decreased,acidic, there is statisticallysignificant difference （P<0.05）.Every groups after electrochemical dose treatment, there is statistically significantdifference among the cathode at pH value （P<0.05）.There is statistically significant differenceamong the anode at pH values changed between the dose of each electrochemical dose （P<0.05）. Howerver,the changes in amplitude of the20C PH value.Compared with the control group, inhibition rates of human breast cancer MCF-7cells intreatment group was gradually increased with the electricity consumption increased（P<0.05）.Compared with the control group, the intervention group increased with the electricity consumption of the intracellular Ca²⁺concentration increasing,and showed the dose-effectrelationship.24hours increased more obviously than six hours.It indicated that ECT couldhinghten intracellular Ca²⁺concentration in MCF-7cells. increased,and the intracellular Ca²⁺concentration and apoptosis was positively correlated. in a manner of quantity of electricitydependent.The treatment group the proportion in the G0/G1phase gradually increased,but theproportion in the S phase gradually decreased （P<0.05）,and the proportion in the G2/M phasewas not obvious（P>0.05）.The cell apoptosis rate at3C、5C、l0C.gradually increased,and at20C was not obvious（P<0.05）. Compared with the control group, the cellular necrosis rate gradually increased（P<0.05）, and24hours increased obviouslier than six hours（P<0.05）.The expression of c-mycand cyclin E increased while the number of positive cells gradually decreased in the treatmentgroup.24h was more significantly than6h in every indexes after the electrochemical treatment.Conclusions The electrochemical therapy with low milli-Ampere（10mA） can changethe PH value of its living environment in the human breast cancer MCF-7cells and improveintracellular Ca²⁺concentration,which can lead to decrease the expression of c-myc and cyclinE and make cells stop in the G0/G1phase,so that it can hamper MCF-7cells growing up andencourage cells apoptosis, which is one of the mechanisms for ECT to cure cancer.However,low doses of electrochemical therapy induce apoptosis and high doses ofelectrochemical treatment cause cell necrosis..