Changes Of Lymphocyte Subpopulations, Interleukin-2 And Cortisol In Peripheral Blood From Patients With Hyperprolactinemia

Objective: 1.To observe the levels of CD4~+T lymphocyte , D8~+T lymphocyte, CD3-CD16~+CD56~+NK cell in peripheral blood of patients with hyperprolactinemia and determine the concentrations of interleukin-2 (IL-2) and anticardiolipin IgG, IgA, IgM in the serum of hyperprolactinemic patients,then investigate the immunal function and it's change rules of hyperprolactinemia. 2. To determin the levels of cortisol (Cs) and adrenocorticotropin (ACTH) and their diurnal rhythm , then investigate the function of hypothalamic-pituitary-adrenal (H-P-A) axis and the changes in the condition of hyperprolactinemia. 3. The levels of sex hormones were tested in patients with hyperprolactinemia in order to investigate the influence caused by high level prolactin. 4. To analyse the correlations between each representative parameter and their changes, then generally evaluate the neuro-endocrine-immunoregulatory network's functions and changes in women with hyperprolactinemia5.Investigate multiple roles of prolactin as a cytokine,an endocrine hormone and aneurotransmitters Methods: Thirty-four hyperprolactinemic patients and twenty normal fertile women were selected in this study. 1.The levels of CD4~+T lymphocyte, CD8~+T lymphocyte, CD3-CD16~+CD56~+NK cell was test with the method of flow cytometre. 2.Enzyme chemiluminescent immunoassays were employed to test the levels of cortisol and ACTH (8:00AM and 4:00PM in the same day) in thirty-four patients with hyperprolactinemia and twenty normal fertile women. 3.Radioimmunoassy was used to test the level of IL-2 in hyperprolactinemic women (pro-therapy and post-therapy) treated with bromocriptine and twenty normal fertile women. 4.Enzyme linked immunoabsorbent assay was used to test anticardiolipin in thirty-four patients with hyperprolactinemia and twenty normal fertile women. 5.The sex hormones including follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone (T) and prolactin (PRL) in the serum were examined with immunoenzymetric assay (magnetic solid phase). Results: 1. The CD4~+T lymphocyte cell is significantly reduced in hyperprolactinemic patients compared to normal subjects (P<0.05). Of all the patients, the CD4~+T level of the amenorrhea group is significantly reduced compared to normal controls (P<0.05), but it is not reduced neither in the tumor group nor the galactorrhea group compared to normal controls (P>0.05, P>0.05).Meanwhile,the level of CD4~+T was not different between the amenorrhea and the non-amenorrhea group, the galactorrhea and the non-galactorrhea group, the tumor and the non-tumor group (P>0.05,P>0.05,P>0.05). 2. The CD3~+ level of the hyperprolactinemic patients was lower and the CD8~+T level was higher both than that of the normal controls (P>0.05,P>0.05), but no significant difference of CD3~+T and CD8~+T levels was found between the patients and the normal subjects respectively (P>0.05,P>0.05). 3. As a consequence of changes in the level of CD4~+T and CD8~+T, hyperprolactinemic patients have a lower ratio of CD4~+T/ CD8~+T than normal control (P<0.05). There are no difference about the ratio between the amenorrhea and the non-amenorrhea group, the galactorrhea and the non-galactorrhea group, the tumor and the non-tumor group (P>0.05,P>0.05,P>0.05). 4. The level of NK cell is not different between hyperprolactinemic patients and normal subjects (P>0.05). 5. Hyperprolactinemic patients have a higher level of IL-2 than that of normal control and so do the amenorrhea group (P<0.01,P<0.05). The level of serum IL-2 (post-therapy) was not significantly different from control group (P>0.05). 6. In hyperprolactinemic patients we observed higher cortisol and ACTH levels at 4:00PM and lower ACTH level at 8:00AM compared to controls (P<0.05,P<0.01,P<0.01).Meanwhile,we observed abnormal secretion diurnal rhythm of cortisol and ACTH.There wasno nomal ACTH peak phase at 8:00AM. The normal rhythm about 2 to 1 (8:00AM to 4:00PM) changed to 1 to 1. We also found a higher ratio of p...