Establishment Of Animal Model With GongHuanChuXue Syndromes And Experiment Study Of Its Pathogenesis

Objective: To search for a method of building up the animal model with GongHuanChuXue(GHCX) diseases and TCM syndromes. Moreover, to study the pathogenesis of GHCX on the model.Methods: Based on the TCM theory, three Cu-IUD-wearing rabbit models of QiXuXueYu syndromes(deficiency of vital energy and stagnation of blood), GanYuXueYu syndromes(depression of the liver and stagnation of blood) and YinXuXueYu syndromes(deficiency of Yin and stagnation of blood) were builded up. Several items such as the change of hemorrheologic, the change of general activity and body weight , the mean weight of main organs ,the ultrastructures of hepatocytes, the cyclic nucleotide acid in plasma and their ratio were observed.Results: (1)Wearing Cu-IUD could bring blood into the hypercoagulable state for it could increase the level of total blood viscosity , plasma viscosity, red cell volume, and accelerate the speed of erythrocyte sedimentation. (2)All animals had the alteration of general activity and body weight lossing in variant degrees.(3)The main organs in QiXuXueYu group all had atrophied in variant degrees, while the GanYuXueYu group and YinXuXueYu group had no obvious change. (7)The hepatocyte ultrastructures in the GanYuXueYu group were impaired, while it hadn't visible change in the QiXuXueYu group and YinXuXueYu group. (5) The level of plasma cyclic adenosine monophosphate (cAMP) in the YinXuXueYu group increased, while cyclic guanosine monophosphate(cGMP) had no obvious change, so the cAMP/cGMP ratio raised. Contrarily, the level of cAMP in the QiXuXueYu group and GanYuXueYu group decreased, while cGMP increased, so the ratio of cAMP/cGMP declined.Conclusion: All above indicated that different model had various phenotype because of the individual difference. The nature of GHCX's pathogenesis was the impairment by the metal foreign material directly and the main pathogenesis of GHCX was the stagnation of blood in vessel. Blood stagnation induced by the IUD was the fundamental etiology of GHCX.