Establishment Of Animal Model With Liver Depression And Blood Stasis Syndrome Of GongHuanChuXue Syndromes And It's Experiment Study

objective:This topic was guided by traditional Chinese medicine to search for a method of building up the animal model with liver depression and blood stasis syndrome of GongHuanChuXue(GHCX:endometrorrhagia which is caused by IUD-wearing) diseases and TCM syndromes.Moreover, to study the pathogenesis of GHCX on the model.Methods:The rats were divided into normal control group, only IUD placement group, model group and treatment group. Liver depression is a multifaceted psychological disorder that involves changes in behavior, immunological function and responses. Make rat Liver depression model by chronic stress on restrictions on the activities and isolate fed.Observe the weight, sucrose preference solution and automatic behavior. Analyze all of the data from experiment. In the present study, we applied a novel proteomic technology, antibody array, to measure the level of uterus cytokines of the rat model. Observe the correlation between weight, sucrose preference and behavior. Discuss the role of cytokines playing in the liver depression and through treatment confirmed the Cu-IUD liver depression rat model is reasonable.Results:There were abnormal behaviors, such as decrease of elusion, slackness, looser stools, and mattefur were observed among model group rats. The body weight of model group was signifi cantly lower than that of control group (P<0.05). Fluid consumption test revealed that model rats lost appetite for sweet water (P<0.05) during the whole procession.comparing with only IUD-wearing group, a total of 4 cytokines in uterus from liver depression and blood stasis with Cu-IUD-wearing group was varied significantly.It was shown that the level of CINC declined significantly compared with the IUD-wearing group, and the level of IL-1β,IL-6,TNF-aincreased (P<0.05).Conclusion:The nature of GHCX's pathogenesis was the impairment by the metal foreign material directly and the main pathogenesis of GHCX was the stagnation of blood in vessel and the syndrome of stagnation of liver qi.This experiment based on etiological, behavioral and pathological observation, it can be concluded that the rat model of syndrome of stagnation of liver qi was established successfully by restrictions on the activities and isolate fed. This studies has shown that antibody array could serve as a fast, high-throughput and sensitive tool for detecting levels of rat's uterus cytokines, and CNTF,IL-1β,IL-6,TNF-αare possibly related with the pathophysiology of syndrome of stagnation of liver qi.