In Vitro Study Of Laser Heart Valvuloplasty And Valvulo--endothelialization

object: to establish a new method of heart valvuloplasty.methods: endothelial cells were obtained from human umbilical vein with enzyme digesting method, and primary culture and subculture of the endothelial cells at the laboratory. The cell growth curve were drawed up, and CD34 and VIII factor antigen were evaluated to confirm the endothelial cell quality. The diseased mitral valves that were harvested from the patients undergoing the operation of mitral valve replacement, were debrided wi th Nd: YAP laser and the cultured endothel ial eel Is were seeded on the surface of laser梚rradiated mitral valves. Then the valves seeded with endothelial cells, were cultured for 2^ 4, 6 and 8 days respectively. The scanning electroscope evaluation was done during these periods. After 8梔ays culture, The valves were undergone a test with modelled device on human blood circulation for four hours. Afterwards, a further scanning electroscope study was taken again.Results: After 2ays and 4ays grafting, the HUVEC (human umbilical vein endothelial cell ) covered 20?0% and 50?0% of surface of the valves respectively; There was a monolayer of seeded HUVEC covering the heart valve surface at the 6 to 8 days. After 4- hours flushing test, there still were 30-40% at the valves surface covered with the endothelial cells. Conclusion: laser valvuloplasty and valvulo?endothelialization were proved to be worthy of further clinical trial.