The Study Of The Relationship Between BMSCs And Cementum Formation In Vitro

New cementum formation was important in periodontal tissue regeneration. In recent years, many studies indicated that culture cells which obtained from periodontal ligament, alveolar bone and bone could form new periodontal tissues after reimplanted in vivo . But these cells were less origin and too difficult to obtain, and it could t achieve ideal results in old patients and in that which periodontal tissues was defect seriously. Bone marrow stromal cells have various differentiation potentials. They can differentiate into osteoblasts, fibroblasts, cartilage cells and adipocyte on special cultured condition in vitro. But formation of periodontal tissues in vitro by bone marrow stromal cells has rarely been reported. The purpose of this study was to compare the difference between BMSCs co-cultured with cementum and bone, to study the relationship between BMSCs and cementum formation in vitro. Material and method: The BMSCs cells were obtained from the femora of SD rats, after subcultured in vitro, for first-subculture and second-subculture cells were used for the experiments . Cells were co-cultured with root slices and femora slices which were obtained from bovine incisor and SD rats femora in minerlization4culture medium in vitro. SEM, histochemical and enzymology determination were used to determin the biological behavior of BMSCs on different slices. Results: l.SEM: The cultured cells in association with cementum slices grow better and attached close to the surfaces at 7th day, Most of the cells were fusiform-shaped and exhibited a cementoblast-like appearance. In contrast, the cells in association with bone were polygon-shaped and exhibited a osteoblast-like appearance. At 28th day, the cells in cementum group were multilayers and with the mineralized nodules formation. The cells in bone group were multilayers and showed minerlization matrix formation. It showed that the matrix of cementum and bone can modulate the behavior of cultured cells. 2^ BMSCs could form cementum-like and bone-like tissues on the surfaces of cementum and bone observed by light microscope. 3^ Both cementum group and bone group have expressed ALP activity, but it was lower in cementum group than that of in bone group(p<0.05). 4 > Bothcementum group and bone group have osteocalcin(OC) expressed, and it was higher in bone group than that in cementum group(p < 0.05). ConclutionrThe results indicated that BMSCs which co-cultured with cementum and bone could differentiate to osteoblasts-like and cementum-like cell. The cells could form cementum-like tissue on the surface of cementum slices.