An Experimental Study On The Effect Of Ginsenoside On The Level Of Serum Albumin And IL-6 IN Rats Affected By Septic Peritonitis

[Objective] Septic peritonitis causes systemic inflammatory response syndrome (SIRS), in which multiple cytokines (CKs) are involved directly or indirectly. CKs not only have various biological functions but also make up a quite complicated network by stimulating, inducing and regulating with each other, to participate in organism's immunity, inflammation, infection and transplant rejection, etc. It is on the basis of this that the theory of Cytokine Storm was put forward to account for the mechanism of SIRS. When SIRS takes place, serum albumin level drops sharply with excessive consumption of energy and excessive catabolism. Does it mean malnutrition or severe inflammation? Recent studies showed that the low albumin syndrome is mainly caused by serum albumin's reaction against diseases and trauma. Therefore the low level of serum albumin cannot be used to reflect the nutrition status of an organism, but a good indicator of the degree of seriousness of systemic inflammatory response. Panax gensing has a bi-directional regulatory function of both organism and metabolism, which helps recover and promote the function of organism, i.e, improve dysfunction caused by both internal and external factors such as stress, drugs trauma.(1) In this experiment models of peritonitis of experimental rats are made by ligating and transfixing their caecum. Ginsenoside solution is perfused into rats a week before the model-making and during the survival period after such model-making. The serum concentration of albumin and IL-6 are assayed and then the effect of ginsenoside on albumin and IL-6 are assessed.[Methods] 40 rats are equally divided into four groups at random: 1) A control group infused only with distilled water 2ml,qd.n=10. 2) A control group infused with ginsenoside solution in 30mg/kg and concentration 0.3%.qd.n=10. 3) A group infused with distilled water and made into models 7 days later. 4) A group infused with ginsenoside solution and made into models seven days later. The four groups areobserved for 96 hours and the survivals are continued to be infused with distilled water or ginsenoside solution. 48 and 96 hours after model-making, all the rats are sampled for blood and dissected for observation of pathologic changes in their peritoneum.The SPSS 10.0 software is adopted for statistic purpose of quantitative data. RESULTS: 1) serum albumin unit: g/LStatistical parameter Experimental data NMeanStd deviationControl group infused with distilled water1030.806.32Control group infused with ginsenoside1027.403.84Operative group infused with water 48 hours917.331.58laterOperative group infused with ginsenoside920.222.6848hours laterOperative group infused with water 96 hours918.002.29laterOperative group infused with ginsenoside 96822.252.38hours laterComparison of the means of serum albuminStatistical parameter Experimental dataNSubset for alpha=0.05Operative group infused with 9 1.2373water 48 hours laterOperative group infused with 9 1.2521water 96 hours laterOperative group infused With 9 1.30241.3024ginsenoside 48hours lateOperative group infused with 81.3450ginsenoside 96 hours laterControl group infused with 101.4339ginsenosideControl group infused with 101.4804distilled waterSig. 0.0720.146 0.113Siudenl-Newman-keuls "?lestAs the above table shows:when a=0.05, the six experimental groups are statistically at three different levels. After operation, the albumin level shows a dramatic decrease in the control group while there is less decrease in ginsenoside-infused group due to the interference of ginsenoside, and the difference is significant.2) serum interleukin-6 unit: pg/ml Statistical parameter Experimental data N MeanStd deviationControl grou...