Clinical Trial And Experimental Research Of Blood Stasis Of Wei-fen Symptom Complex In Seasonal Febrile Disease

Purpose: To investigate whether there occurs blood stasis during the stage of Wei-fen symptom complex in seasonal fehrilc diseases, and establish guideline for clinical practice. Method and Result: A control led trial was conducted: 32 patients suffering Wei-fen symptom complex were screened. The concentration of endothel in-1 (ET-1), 6-keto-PGF1, and thromboxaneB2(TXB2) were measured by radioimmunoassay(Rl.A), and the level of D-dimer(D-D) were tested by enzyme-linked immunosorhent assay(ELISA).Results: the concentration of ET-1, 6-K and TXB2 were significantly higher than those of the controlled groups, and the difference between the rate of TXB2/6-K and that of D-D didn't show statistically significant. In order to investigate the reason of the increase of ET-1, 6-K and TXB2 and whether there are different changes of these markers accompanying different degree of Wei-fen symptom complex, a randomized, controlled experiment was conducted. 32 tibbits were randomly divided into ordinary Wei-fen group (A), serious Wei-fen group(B), controlled group(C)and controlled group in high temperature environnient(D). The rabbits of A and B groups were venously injected different dose of endotoxin to establish different degree of Wei-fen symptom complex, and the rabbits of D were put in the incut)ator with temperature regulating setting to keep their temperature identical with those of A groups. The level of ET- 1,6-k,and TXB2 were measured by sampling blood, at baseline, immediately after establishing Wei-fen symptom complex models, the U line of. tcmperaturc of A group rabbits rocoveri rig to normal and the time of 24 hours after the temperature of A group rabbits recovering to normal level. After the fourth blood satnpl ing, all rabbits were sacrificed to analyze pathologic changes. Results: 1.The changes of ET-1: no difference at the baseline; the ET-1 level of B group increased (compared with A, C, D, P<0. 01) after second blood sampling; the level of A group increased (compared with C, P(0. 05) and the level of 13 group was hi giter than thosc?of C and I) groups (l^{0. 05} after th i rd sampling; after fourth sampling the level of B declined but still higher than the levels of other groups (P(0.05), and the levels of A and D groups didn't show significantly different through the experiment. 2. The changes of 6-k and TXB2: no difference among all groups at baseline; the levels of 6-k and TXB2 of A, B and D groups increased after second sampling (compared with C, P<0.01), and the level of B group increased significantly (compared with A and 1), P<0. 01) ; the levels of 6-k and TXB2 of A, B and P groups remained high and the level of B was still higher than those of A arid D (P<0. 01) after third sampling; at the last sampling, the levels of 6-k of A, B and D groups declined to normal level and the level of TXB2, of B was statistically hi ghe r titan those of?other groups. 3. The changes of T/k: there was no difference among A, C and D through the experiment; the value of T/k of B group increased higher at the time of second sampl ing than other t i.tnes (P<0. 01), and higher than other groups (P<0. 01) (at the same time of satnpl ing). At the third and fourth time of sampling, the value of T/K of B group still remained high (compared with the baseline, P<0. 01 ;cotnpared with those of other groups at the 2 saute time,...