Study On The Technical System Of Tissue Culture And Rapid Propagation Of Bauhinia Blakeana

The study was mainly to probe an efficient way through tissue and rapid propagation of Bauhinia blakeana. The stems with shoot, the leaves, petiole were taken as explants. For know the better medium and proceeding of culturing Bauhinia blakeana., we used orthogonal experimental design and analysis of variance to establish the technique system of tissue culture. The affected factors of propagation, existing problems in this technique and the corresponding solutions was also described, which provide the theoretical base and technical measures for industrial producing of plantlet.The study of tissue culture technique and the better medium for various stages are as follows:①March is the best season to draw materials, materials, in which the germination rate are 91.6%,81.8% and 89.5% by stem, laminae and petiole respectively, the contaminated rate only are 21.6%,19.3% and 19.6% by stem, laminae and petiole respectively.②During the trial:8 minutes is good to treat the stem by HgC12 (0.1%),4 minutes is good to treat the laminae, and 6 minutes is good to treat the petiole.③Initial medium of stem:MS+6-BA 2.0 mg.L-1+NAA 0.01 mg.L-1+IBA 0.5 mg.L-1 in which the germination rate is 91.4%, the callus induction rate is 81.2%, and the shoot forming index is 1.8; Initial medium of aminae:MS+6-BA 1.0 mg.L-1+NAA 0.1 mg.L-1+2,4-D 0.1 mg.L-1,in which the callus induction rate is 75.6%; Initial medium of petiole:MS+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1+2,4-D 0.05 mg·L-1,in which the callus induction rate is 71.8%.④Proliferating of stems cultivation:The optional proliferating buds culture medium stems and shoots was:MS+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1+IBA 0.5 mg·L-1,in which the multiplication rate is 3.7 and the multiplication of efficiency seedings is 2.7.Highly proliferating callus was obtained following cultivation on MS+6-BA 0.5 mg.L-1+NAA 0.1 mg.L-1+2,4-D 0.5 mg.L-1.The proliferating rates could be up to 4.4.The best culture medium for callus of stems differentiating was MS+6-BA 1.0 mg.L-1+NAA 1.0 mg.L-1.The regeneration rate was 85.9%. ⑤Efficiency seeding culture:MS, in which the rate of efficiency seedings is 85.9%.⑥Rooting culture:MS+IBA 1.0 mg.L-1 in which the rate of rooting is 76.8%, the quantity of roots is 3.3, and the length is 5.1 cm.⑦Forging seeding transplanting:The transplant medium in vermiculite, perlite and riversand by 1:1:2 is the optimal for Bauhinia blakeana., which the rate of survival is 73.5%.