The Research On Tissue Culture Of Pear

The tissue culture of the pear has been researched systematically. Used the stems of Cuiguan pear, the embryo of 13 different combinations of pear hybridize and self-breeding leaves of Niitaka pear as explants, the basic conditions of culture and some essential factors of regeneration have been determined. In addition, the transplants of plantlet have been researched.All of these researches are benefit to the foundation of pear tissue culture and its implication on rapid propagation of fine varieties. The main results are shown as follows:1, embryo culture: With the medium of 1/2MS+6-BA0.5mg/L, the embryo are easy to regenerate plantlet. At the same medium,the different capabilities of regeneration have been observed on different combinations of pear hybridize. Compared to other combinations ,the combination of Pyrus pyrifolia and Pyrus pyrifolia have the higher buding and rooting frequency at the medium above. Moreover, the pear embryo with part of cotyledon can grow better than the pear embryo without cotyledon or the pear embryo with whole of cotyledon.2, stem culture: Under the hormone level of 6-BA2.0mg/Land IBA0.2mg/L, the regeneration ratio on the basic medium of 1/2 MS is higher than on the basic medium WPM or AS . The stem with buds can regenerate plantlet directly without dedifferentiation, but the stem without buds must be induced callus firstly. According to the three basic medium of this research, AS adapts to the no bud stem culture, and the callus are health, but it need look for another dividing medium.3, leaf culture:After orthorhombic test, the NN69+0.5 mg/1 TDZ+0.3 mg/1 IAA has been determined as the best medium. In addition, it can obtain the highest callus frequency and budding frequency with inverted explanting of leaves. Subctltured different ages of leaves regenerated from embryo culture,both callus frequency and budding frequency of 15-30-day-old are much higher than 30-day-old4, transplant: the technique of transplant has been researched. The result shew that the plantlets grew well no matter what transplant method. The result indicated that the important stage which determine tissue culture whether or nor success is at tube-stage, and the transplant methods only affects the long of plantlets to adapt to growth condition.