Preparation Of The "Xiao Qing Ying" Injection And Study On Main Pharmacodynamics

Confirmed the febrile infectious disease caused by causative organism of swine as febrile disease, according to Chinese traditional veterinary theory. Decided to curatio this disease during the transmission of "Qi" to "Ying" with "Qing Ying Tang" which can prevent the transmission.Utilized LPS to model the transmission of "Qi" to "Ying" of rabbit. And evaluated the curative effect of "Qing Ying Tang" taken apart in order to reduced it on this model. The result was that the effect of Radix Rehmanniae, Radix ScropHulariae, Radix Salviae Miltiorrhiae, Flos Lonicerae and Fructus Forsythiae could equal "Qing Ying Tang" in aspects of relieving fever, disengaging blood stasis and calming the nerves.Abstracted effective constituents from Radix Rehmanniae, Radix ScropHulariae, Radix Salviae Miltiorrhiae, Flos Lonicerae and Fructus Forsythiae using supersonic wave. Designed orthogonal test to sieve the best conditions of abstraction. The result was that the best condition of abstracting together general chlorogenic acid and Salvianolic acid B from Flos Lonicerae and Radix Salviae Miltiorrhiae was that soaked medicinal substances in 40% alcohol Liq.(pH=3) which wasl5 times more than them 24 hours, then abstracted them 45 minutes; The best condition of abstracting together catalpol and forsythin from Radix Rehmanniae, Radix ScropHulariae and Fructus Forsythiae was that soaked medicinal substances in 70% alcohol Liq. which was 20 times more than them 12 hours, then abstracted them 30 minutes.Mixed foregoing two extracts up to manufacture injection and designed test to find the best parameter of manufacturing technique. The result was that adjusted pH of mixed liquor to 6.5, add alcohol into, made extreme concentration was 10%, then added 0.2%VC and 0.05% EDTA-2Na into. Named it "Xiao Qing Ying" injection.Evaluated the main pharmacodynamic action of-"Xiao Qing Ying" injection on the model of transmission of "Qi" to "Ying" of rabbit. The result was that this injection possessed conspicuous actions of relieving fever, disengaging blood stasis, calming the nerves, counteracting oxidative damage and correcting the disce of electrolytes in blood serum. These pharmacodynamic actions relied on dosage.