The Prokaryotic Diversity And Biogeography In The Hot Gas Spring Soils,xinjiang By Culture-independent Approach

In order to investigate the prokaryotic diversity and biogeography in the hot gas spring soils, Xinjiang by Culture-independent Approach, we constructed bacterial and archaeal 16S rDNA clone libraries and then analysed the bacterial and archaeal diversity of the hot gas spring in MaNasi country. The aprA gene products of sulfate-reducing and sulfur-oxidizing prokaryote and bacterial 16S rDNA genes were analyzed with Terminal-Restriction Fragment Length Polymorphisms method and then studied the relationship between the Shannon-index and environmental factors. The main results were as follows:(1)170 positive clones were randomly sleceted from the Bacterial 16S rDNA clone library,then processed by restriction fragment length polymorphism(RFLP) method using Haeâ…¢. Unique rDNA types clones were sequenced, analysed and then constructed phylogenetic tree. Twenty-nine operational taxonomic units (OTU) from 170 positive clones were found belonging to 6 phyla: Firmicutes, Proteobacteria, Acidobacteria, Bacteroidetes, Planctomycetes, Actinobacteria. Firmicutes (71%) was the absolutely dominant components of the soil bacterial community. 14 sequences (about 1.5 kb) from 29 OTUs showed less affiliation with known taxa(<97% sequence similarity).Soil bacterial diversity is low in the hot gas spring soil, but exit a large number of new unknown taxon in this environment.(2) 98 positive clones were randomly sleceted from the Archaeal 16S rDNA clone library,then processed by restriction fragment length polymorphism(RFLP) method using Hhaâ… . Unique rDNA types clones were sequenced, analysed and then constructed phylogenetic tree.Sixteen operational taxonomic units (OTU) from 98 positive clones were found belonging to Crenarchaeota and divided two groups. Crenarchaeota was the absolutely dominant components of the soil bacterial community inhabited in the hot gas spring soils.(3)The PCR products of aprA gene from four hot gas spring soils were analysed by Terminal-Restriction Fragment Length Polymorphisms (RFLP) method with two different kind of restricted enzyme(Haeâ…¢and Hhaâ… ). Combined with the environmental parameters by statistical analysis, investigated the relationship between the biodiversity of sulfate-reducing and sulfur-oxidizing prokaryotes inhabitating in the hot gas spring soil and environmental factors, the location of the sampling sites. The results revealed that the biodiversity just only was related to the temperature and pH of soils. So we can forecast the the biodiversity sulfate-reducing and sulfur-oxidizing prokaryotes in soils according to the temperature and pH of the four hot gas springs soils in a certain range.(4)The products of bacterial 16S rDNA from four hot gas spring soils were analysed by Terminal-Restriction Fragment Length Polymorphisms (RFLP) method with two different kind of restricted enzyme(Rsaâ… and Hhaâ… ).Combined with the environmental parameters by statistical analysis, investigated the relationship between the biodiversity inhabitating in the hot gas spring soil and environmental factors, the location of the sampling sites. The results revealed that the biodiversity of bacteria was related to the environmental factors except temperature and pH.