| With the continue expansion of the Equestrian industry in Shanghai, improvement of quality horse care, disease control and prevention are in demand, these open up a whole new topic for the veterinary researchers. On the preliminarily study, the writer summarized their findings that the characteristics of the Shanghai's climate plays an important part. This is the first time using horses in the study, besides improve the feed and sanitation condition, Chinese herbal medicine are to be added to the feed. The objective is to set up a reasonable disinfection plan and at the same time observe the effect of Chinese medicine on horses immune system and deworming. The results showed that:The first experiment using microbiology method to analysis distribution of pathogenic micro-organisms, before and after disinfecting the stable stalls. Distribution of micro-organisms showed a certain upward trend at different period. The day after disinfection, number of Fecal Bacteria in the feces significantly lower (P <0.05), changes were not significant on Day 1-3 (P> 0.05). Day 4 bacteria significantly increased, Day 6 bacteria back to the same as before disinfection.The second experiment : after performing vivo tests on fresh samples of horse stool, result of the herbal group showed that the parasite egg count had an upward trend during week 1 and 2 after treatment. On Week 3 - 6, the count was significantly lower. The result from the control group showed the egg count had a downward trend, however it was basically stayed the same. The endpoint nephelometry, RT-PCR method was used to determine impact of Chinese herbal medicines additives in the horse's blood IgG and cytokines IL-2, IFN-γlevels. The results showed significant difference in the treatment group horse serum IgG content compared with the control group (P <0.05), To some extent, Chinese herbal medicine as feed additives can increase horse blood levels of IgG; both groups ofβ-actin mRNA were consistant. The treatment group IL-2 mRNA increased during Week 3 and 4, and IFN-γmRNA with no change.All above results indicated, scientific disinfection method has a positive effect on pathogenic diseases prevention. Adding proper amount of Chinese Herbal medicine additives to the horse diet could significantly improve their immune system and inhibit intestinal pathogenic microbes and worms of horses. |
