Effects Of Exogenous Substances On In Vitro Plantlet Regeneation Of Different Ploidy Paulownias Seedlings And Their Protein Changes

The pawlownia is one of our country important fast-growing timber wood tree seeds, is improving our country ecological environment and raises the people's living standards.,but People are seriously affected the enthusiasm of the cultivation of Paulownia. Paulownia callus induction is as a result of a short time and in the same cycle of callus to split more conducive to study physiology and biochemistry;To study the transcription of DNA methylation inhibitors (5-Aza) and histone- deacetvlase (4-Phenylbutyric acid, Chaps) on the Growth of Paulownia, the experiment with using two-dimensional gel electrophoresis analysis of proteins after 5-Aza, 4-Phenylbutyric acid, Chaps treatment on P. fortunei diploid and tetraploid P. fortunei differences in gene expression product; study of 5-Aza, 4-Phenylbutyric acid ,Chaps, in cell dedifferentiation process role, and then explore the DNA methylation inhibitor (5-Aza) and histone-deacetvlase acetyltransferase inhibitor (4-Phenylbutyric acid, Chaps) of Paulownia leaf bud induction, seedling morphology, protein expression results, Learn eventually 5-Aza, 4-Phenylbutyric acid, Chaps on the growth and development of Paulownia. The main findings are as follows:1 Inhibition of exogenous substances in vitro plant regeneration of Paulownia 5-Aza, Chaps, 4-Phenylbutyric acid, treatment P. fortunei diploid and tetraploid and tetraploid has in vitro inhibitory effect of plant regeneration, and with 5 -Aza, Chaps, 4-Phenylbutyric acid, enhanced inhibitory concentration increased.2 Exogenous substances on seedling have effect on morphological changes With 5-Aza, Chaps, 4-Phenylbutyric acid reatment on P. fortunei diploid and tetraploid and tetraploid morphology differences must exist, with 5-Aza deal with the Paulownia appeared numerous buds and plant dwarfing, 4-Phenylbutyric acid treatment of both the roots of Paulownia have substantial callus, with 5-Aza, Chaps, 4-Phenylbutyric acid concentration increased, seedlings, the more obvious symptoms. With Chaps paulownia seedlings of three treatment patterns did not change significantly.3 Exogenous substances on diploid and tetraploid P. fortunei seedlings the leaves have effect on proteins Leaves of seelding by 5-Aza, Chaps, 4-Phenylbutyric acid to deal with found in two-dimensional gel electrophoresis protein isoelectric point, molecular weight, protein content, the same species of different concentrations of exogenous substances to deal with the difference between the presence of seedlings in the treatment of seedlings differences exist. After dealing with 5-Aza diploid P. fortunei seedlings, protein PI 6.6, 22KD (a), PI 6.5, 21.8KD (b), PI 5.4,14.5 KD (k) to enhance the expression of protein PI 5.2,17.3 KD (e ), PI 5.2, 16.7KD (f) reduced expression; while dealing with 5-Aza tetraploid P. fortunei leaf protein PI 6.6, 22KD (a), PI 6.5, 21.8KD (b) a gradual diminuendo; protein PI 5.2, 17.3 KD (e) and PI 5.8, PI 5.2, 16.7KD (f) until the disappearance of warning. Paulownia leaves handling by the 4-Phenylbutyric acid appear diploid-specific protein 5.2,17.3 KD (e), 5.8,16.5 KD (o); by 4-Phenylbutyric acid handling protein tetraploid P. fortunei leaves PI 6.6, 22KD (a), PI 6.5, 21.8KD (b), PI 5.2, 16.7KD (f) is the difference in protein. Chaps by P. fortunei leaf protein processing can be found, with the increase in protein. Chaps concentration PI 6.4,20.1 KD (p), PI 6.56,19.8 KD (q) gradually increased, and the emergence of differences of protein PI 5.2,17.3 KD (e); chaps After dealing with tetraploid P. fortunei leaf protein two-dimensional gel electrophoresis protein PI 6.6, 22KD (a), PI 6.5, 21.8KD (b) enhanced after the first weakened, PI 5.2, 16.7KD (f) has been reduced...