Study On The Effect On Different Kinds Of Paulownia In Vitro Regeneration With Methyl Methanesulfonate

First, the tissue culture’s leaves of P. tomentosa, P. fortunei and P. tomentosa×P. fortunei were used as materials in this experiment. In their leaves’regeneration proliferation medium of Paulownia the different concentrations of methyl methanesulfonate(MMS) respectively were added for the regeneration. Second, the tissue culture seedlings of three kinds Paulownia were used as materials, the different concentrations of MMS were added in 1/2 MS medium to study for the changed of Paulownia. Then their global DNA methylation levels were detected to study on the relationship between their global DNA methylation levels and MMS treatments. These were based for the theoretical and technical basis of the Paulownia DNA mutation breeding new varieties and Paulownia improvement. In this paper main results are as follows:1, It was a negative relation between the induction rates of leaves callus and the global DNA methylation levels of P. tomentosa leaves callus. The buds differentiate rates used the leaves’regeneration of P. tomentosa decreased as the increasing concentration of MMS. The rates of every treatment with MMS were significant. When the concentration of MMS reached 200 mg ? L-1, there was no buds to induce. In the 1/2MS media the root induction rates decreased with the increasing concentration of MMS, and the numbers of roots became few, and the rates of rooting decreased, and the growth of roots was inhibited.2, It was a negative relation between the induction rates of leaves callus and the global DNA methylation levels of P. fortunei leaves callus. The buds differentiate rates used the leaves’regeneration of P. fortunei decreased as the increasing concentration of MMS. In the 1/2MS media the root induction rates decreased with the increasing concentration of MMS, and the numbers of root became few, and the rates of rooting decreased.3, It was a negative relation between the induction rates of leaves callus and the global DNA methylation levels of P. tomentosa×P. fortunei leaves callus. The buds differentiate rates used the leaves’regeneration of P. tomentosa×P. fortunei decreased as the increasing concentration of MMS. The rates of every treatment with MMS were significant. In the 1/2MS media the Root induction rates decreased with the increasing concentration of MMS, and the numbers of root became few, and the rates of rooting decreased, and the growth of roots was inhibited.4, The seedlings morpha of three Paulownia were changed by treatment with MMS. Their stems got shorter by comparison with control in their stems with the different concentrations of MMS. The leaves of Paulownia got longer with the increasing concentration of MMS, and the color of leaves became pale green from green or dark green. The seedlings growth of were inhibited by MMS. The global DNA methylation levels of three Paulownia seedlings increased with the increasing concentration of MMS on the 30 day. The root induction rates of Paulownia decreased with the increasing concentrations of MMS, and the number of roots got few. The roots became coarse, and no fibres. It was a negative relation between the global DNA methylation levels of Paulownia and rooting rates of Paulownia.