| Paulownia is an important fast-growing timber and landscaping trees, by the broad masses of the favorite, with fast-growing, widely distributed, fine materials, such as excellent propagation characteristics easily. To develop the cultivation of Paulownia wood to ease the shortage in our country, improve the ecological environment and export have important social significance, economic significance and ecological significance. However, due to the large number of witches occurred in the production of Paulownia caused very serious harm caused by the enormous annual economic losses. Therefore, the Paulownia witches forestry production has become one of the issues that need to be addressed urgently. Habitat dependent territories from Japan found a second-class paulownia witches disease pathogens - since phytoplasma, scholars at home and abroad in different ways paulownia witches of the original body of bacteria and fungal agents of transmission, host resistance and disease resistance induced by identification, and disease control methods such as a large number of studies have proved that as a result of phytoplasma invasion, Paulownia tree in vivo changes in gene expression, which led to a series of physiological and biochemical disorders of metabolism, which eventually led to the Arbuscular the emergence of disease symptoms. Has been caused by the initial clear mode of transmission of disease pathogens, the occurrence of pathways and pathogenesis of the physiological and biochemical changes.This experiment to patients suffering from paulownia witches miscellaneous Yu Miao One paulownia tissue culture for the study.Paulownia group 1 protein extraction method. The use of three different methods of patient suffering from paulownia witches Yu miscellaneous tissue culture paulownia seedlings One group protein extraction, compared three different methods of protein extraction yield, purity and integrity of methods to draw a group of proteins extracted a rate of 0.285μg·g-1; group protein A260nm/A280nm value of 0.9313, using SDS-PAGE slab discontinuous polyacrylamide gel electrophoresis analysis of strip clear. Methods to determine the best one for the group of protein extraction method.2 rifampicin for patients suffering from paulownia witches miscellaneous Yu Miao One paulownia tissue culture the effects of histone component. Different concentrations of rifampicin treatment on patients suffering from paulownia witches miscellaneous Yu Miao One paulownia tissue culture histone components have a certain influence, the use of rifampicin concentration of 100 mg·L-1, 150 mg·L-1, 200 mg·L-1 to deal with patients suffering from paulownia witches Yu Miscellaneous One tissue culture paulownia seedlings, growth after the extraction of histone 30d, through the analysis of protein electrophoresis, molecular weight of histone H2B to deal with the concentration of rifampin decreased, molecular weight of histone H4 with the rifampicin concentration increases.3 methyl methane sulphonate （MMS） of the patients suffering from paulownia witches miscellaneous Yu Miao One paulownia tissue culture the effects of histone component. Different concentrations of methyl methane sulfonate （MMS） treatment on patients suffering from paulownia witches miscellaneous Yu Miao One paulownia tissue culture histone components have a certain influence, the use of methyl methane sulfonate （MMS） concentration of 20 mg·L -1,40 mg·L-1, 80 mg·L-1 to deal with patients suffering from paulownia witches miscellaneous Yu Miao One paulownia tissue culture, growth 30 d after the extraction of proteins, through protein electrophoresis analysis, molecular weight of histone H2B With the increasing concentration of rifampin decreased histone H4, H2A weight with the rifampicin concentration increases. |
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