The Japanese Potato High Effect Rebirth Systematic Foundation

With the emergence of new varieties of potato,as well as applications in the production of promotional,due to differences in genotypes,and its rapid propagation of virus-free culture system and conditions for the formation microtuber was a significant difference.In this experiment,The Japanese potato strain 06-10 as a test material to explore conditions about its internode culture and proliferation, systematically studied the Japanese potato high effect rebirth systematic foundation and microtuber induction.Set up rapid propagation system of Japanese potato and study the impact of regulation and control factors of microtuber vitro Rapid Propagation.a number of cultural plantlets and microtuber had been obtained Successfully.The results offered the materials and experimental basic and science reference for the microtube in the future research work.The experimental results showed that:1.The combination of 0.1%HgCl₂ 7min+75%C₂H₅OH 15s was suitable the buds on Japanese potato,the contamination rate was 3.16%.The optimal induction medium was MS+CPPU 0.15mg/L,MS+6-BA 1.5mg/L,MS+NAA 0.1mg/L.The rate of buds differentiation was 81.25%.2.Take the Japanese potato 06-10 as the body material,adventitious bud formation through the organogenesis way induction.Discussed the different hormone, the different hormone combination,sucrose density processing and so on internodal has not decided the influence to the Japanese potato 06-10 which the bud formed.The result indicated that,(1) The most appropriate single-factor-induced culture medium of differentiation of adventitious buds was MS+CPPU 0.15mg/L,MS+6-BA 1.5mg/L, MS+NAA O.1mg/L.The adventitious buds differentiation rate were 100%and 94%, But GA induction of adventitious buds showed irregular changes,and CPPU function strongly to 6-BA,GA.(2) Two-hormone combination of estrogen was more favorable than the single factor in culture of potato.Cultured in low concentrations of NAA(0.1mg/L) with the appropriate concentration of CPPU(0.15mg/L) matched, high concentrations of GA(1.5mg/L) with the appropriate concentration of CPPU (0.15mg/L) matched were favorable for growth and could improve the overall quality of seedlings.(3) The effect of sucrose concentration with 30g/L in vitro cultivation had optimal effet;Appropriate concentration of CPPU,NAA,GA combination in vitro potato cultivation subculture significant proliferation;The suitable concentration of CCC(200mg/L) could increased the thick of stem,shorten the range of stem.3.Microtuber induction.(l)Different hormone combination gave different reponses to the effects of microtuber induction.The optimal hormone combination for microtuber induced effects of hormone combinations NAA+CPPU+GA was best on vitro minituber,cytokinin played a very important role in the formation of tubers,GA was considered as inhibitor in the formation and growth of potato,was an important regulator of formation of vitro minituber;400mg/L CCC+CPPU 0.15mg/L could promote early seed potatoes,the number of single bottles of minituber in vitro and seed-setting rate were significant increased.(2)Sugar with 8%concentration was adandtage to the microtuber induction.(3)The culture of 8h light wasbest ways to microtuber induction.4.Induced adventitious root.To explore the effects of different concentrations of CPPU,6-BA,GA,NAA and composition ratio to the influence which the root formation.The results showed,(1)The most appropriate single factor medium of root was MS+NAA1.0mg/L,the rooting rate was 100%;(2)The effect of Multi-factor-induced was better than that of Single-factor-induced on potato seedlings invitro rooting,the average rooting rate was 92.19%;The effect of root induction was notable when added the suitable concentration of IBA to the medium.In the culture of root induction and sound seeding,the optimal medium was MS+IBA 0.5mg/L+ CPPU0.1mg/L+NAA1.0mg/L+GA1.0mg/L,the best training time was 10-40d.5.Transplant potato seedlings in vitro.survival rate of transplanted was 93.50% that was high with the vermiculite.