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Study On Drought Resistance And Screening Of Drought Resistance Identification Indexes In Foxtail Millet At Germination Stage And Seedling Stage

Posted on:2010-06-21Degree:MasterType:Thesis
Country:ChinaCandidate:Y BaiFull Text:PDF
GTID:2143360275964715Subject:Cell biology
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1.49 foxtail millet varieties were provided to study on drought resistance The morphological,growth and development indexes of drought resistance were tested and analyzed during bud and seedling stages.Using the relative magnitudes of representative varieties which had different drought resistances, the morphological changes of plants,physiological functions and biochemistry characteristics,and their relations with drought resistance were studied.The following three section concludede the core points of this study.(1).Plant growth was inhibited under water stress.During bud stage,germinative force and germinative rate were inhibited,and the bud length,coleoptile length,embryonic-root length,and dry matter were decreased accordingly compared with controls.Sprout index of drought resisting was an important index for identification of drought resistance in bud stage,and it was significantly correlated with germinative rate,germinative force,root dry weight,bud dry weight and the transformation percentage of stored substance.As a result,these five indexes could be used as the indexes for identification of drought resistance.Based on principal components analysis,germinative rate and bud length could be used as the indexes for identification of drought resistance.(2).There were conspicuous changes on physiological metabolism in plants under water stress.The soluble sugar,soluble protein,MDA,SPAD,RWC,Pro content changed.The survival percentage of seedling under repeatative treatment and drought was an important index for identification of drought resistance in seedling stage,and it was significantly correlated with RWC,MDA,SPAD.As a result, these three indexes could be used as the indexes for identification of drought resistance.2.In the stress of 20%PEG(treating time:0 h,2 h,4 h,6 h and8 h )and in the way of half quantitative reverse transcription polymerase chain action(RT-PCR) Which actin is the inter-reference,the expression of Dnaj and glyceraldehydes-3-phosphate dehydrogenase sequence is higher in drought than in normal...
Keywords/Search Tags:Foxtail Millet, Sprout index of drought resisting, germinating rate, identification indexes, RWC, MDA, SPAD, Dnaj, glyceraldehydes-3-phosphate dehydrogenase sequence
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