| It has been7000years since Chinese cultivated the drought and infertility tolerant crop, foxtail millet (Setaria italica L.).It originated in China and is distributed in arid and semi-arid areas of North China. There were studies of drought tolerance mostly on evaluatingmethods and morphological features such as phenotypic characteristics, but there is absence of its molecular mechanism and research to explore the related genes. This thesis studied the difference in drought tolerance among50foxtail millet accessions and the expression patterns of drought related genes in drought tolerance and sensitive accessions, under natural drought and PEG stress. The main results are as the followings.(1) Natural drought stress and20%PEG-6000stress were applied to investigate the drought tolerance among30foxtail millet accessions in artificial climate chamber. The results showed there was large difference between the two methods, and PEG stress was more similar to filed results.(2) Fifty accessions were used to study the drought tolerance in different growth stages in rain protection shed. The results showed Hongmujiju was drought tolerance, and different accessions showed different responses in different growth stages.(3) Gene expression profilingshowed there were1465and909genes specifically expressed in drought sensitive and tolerance accession, respectively, and940genes showed drought induced expression.(4) The transcript of drought related gene SiABF3.1and SiABF3.2were decreased and then declined in response to PEG stress, whereas SiPDR5were declined and then decreased, and the transcript of SiNF-YBl declined and then decreased in the drought tolerance accession, whereas decreased in the drought sensitive accession. |
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