| The present study was carried out in order to investigate the effectiveness of the Chinese Herbal formula Jade-screen Powder (Yu Ping Feng San) on immune responses to infectious bursal disease vaccine in chickens. Birds vaccinated with Infectious Bursal Disease Vaccine were fed with Jade-screen Powder at high (A) (1%), middle (B) (0.75%), low (C) (0.5%) dosages. And some birds were allocated as control group one (D) without herbal treatment and control group two (E) without vaccination nor herbal treatment. Peripheral blood samples were taken on days 21, 28, 35, 42 and 49 from ten chickens in each group to determine the level of antibody to IBDV. T cell subsets of CD4+ and CD8+ were determined by flow cytometry at the 21st and 35th days. Phagocytosis activity of macrophages was determined with the experiment of carbon particles clearance at the 21st, 35th and 49th days. The quantities of IFN-γand IL-2 in sera were determined at the 21st, 28th and 35th day. The end pieces of duodenum and jejunum were taken at the 21st, 28th and 35th day, and then made into paraffin wax section. The numbers of IELs were examined by HE staining, GCs by PAS and MCs by TBS. The results suggested that Jade-screen Powder could increase the level of antibody to IBDV. The high dose herbal medicine group manifested higher level of the antibody than that of other groups, and after 28th day significantly. The middle and low dosage groups had the higher level compared with the control groups. Jade-screen Powder could increase the density of CD4+ T cell and the ratio of CD4+/ CD8+ significantly. At the 21st day there was a significant difference between the high, middle and low dose groups; extremely significant difference between groups H, M and control groups. At 35th day there was a significant difference between the high dose group and other groups. Jade-screen Powder also could increase the phagocytosis activity of macrophage and compared with other groups the group high dosage had significant effect. At the 21st day, the quantities of IFN-γin serum in group A, B and C are significantly higher than that of control groups. And the quantities of IFN-γin sera in group A and B were significantly higher than those of group C, D and E at the 28th day. At the 35th day, the quantitiy of IFN-γin sera in group A was still extremely higher than those of other groups. One week after the chickens being vaccinated, the IL-2 quantity in sera of group A was extremely higher than that of other groups. At the 28th and 35th day, compared to other groups, group A and B had higher quantity of IL-2 in sera. At the 21st day there were extremely larger number of IEL in intestinal mucosa from group A, B and C, compared with group D and E. And at the 28th and 35th day there was significantly larger number of IELs in group A than that in group D and E. 7 days after the birds were vaccinated, the number of GCs in group A was significantly larger than that in other groups. 14 and 21 days after the birds were vaccinated, the number of GCs in group A was larger than that in group D and E, and was significantly larger than that in other groups. Concerning the number of MC in the end piece of duodenum, at the 21st day group A and B were extremely significant higher than that in other groups, group A was significantly higher than group B, and at the 28th day group A was significantly higher other groups, group B and group C both were significantly higher than group D and E, but there was no difference between group B and group C, and at the 35th there was significant different among all the groups, and decreased at the sequence of A, B, C, D and E. There were fewer MC in jejunum than in duodenum. And at the 28th day the number of MC in group A compared with that in other groups, the number of MC in group B and C compared with that in group D and E, were higher. And at the 35th there was significant different among all the groups, and decreased at the sequence of A, B, C, D and E. In conclusion, Jade-screen Powder can enhance both the humeral and cellular immunity in chickens challenged with infectious bursal disease vaccine.
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