| Bamboo shoots are bamboo plants in the soil or the underground stem germination which is not just unearthed or exposed ground without sunlight,our country's bamboo resources are the most abundant in the world,bamboo shoots are also important forest resources in our country,and it is also food resources which has highly nutritional and edicinal value.Bamboo shoots canned food factory will process bamboo shoots into can in many provinces of south of changjiang river,and the cans are the best-selling in Southeast Asia and Western European countries.However,there are 40～50%tail materials as the waste materials were transported to countryside,The ratio of used and discarded is 5.5:4.5,it is not only a waste of resources but also is pollution of the environment.It is dead against serious waste material,small content of scientific and technological and low economic status,in order to use of recycled resourcse,improve the utilization of bamboo resources,improve the processing of bamboo shoots social and ecological benefits,this reserch use fresh bamboo shoots tail matrrials as raw material,which is provided by Huizhou bamboo shoots food processing plants in Anhui Huangshan City.It will adopt to comprehensive development of the peroxidase with its juice which was direct squeezed,it will obtain bamboo shoot peroxidase by ammonium sulfate precipitation,acetone precipitation and CM-52 chromatography after reclaiming·F-1 and F-2 protein,furthermore,some enzymology properties have studied.At the same time,with bamboo shoots shell and tail materials as raw materials, extract flavonoids from them by ultrasonic,purified flavonoids with macroporous resin and preliminary determination of the spectrum to purified flavonoids.and then testing its anti-oxidation of vegetable oil by the "Iodine a sodium thiosulfate titration",it can provide to technical methods as anti-oxidants.Experimental results show that the peroxidase's RZ value can get to 3.1,the total protein and the total activity of original squeezing juice are 193.3 mg and 7054.93 U respectively,the total protein and The total activity of the end products are 4.5 mg and 1975.4 U respectively,the ratio of purification is 12 times and the ratio of reclaim is 28%and the yield is 9mg/kg.The results of peroxidase properties show that the optimum temperature is 55℃,its properties is stability under 70℃,there is a slight activation at 40℃and 50℃,its activity will rapidly decrease after 70℃,the activity will riser with temperature before get to the optimum temperature.So the thermal stability is upper.And its optimum pH is 6.8.Their activity is still 80%of the original activity,which was dealt with 24 h at 4℃under pH of 6 to 9,so its stability of pH range is wide.And at process of the peroxidase purification,there can be expected F-1 protein 2.8 mg/kg,F-2 protein 3.8 mg/kg,the former can be as additives for food or feed,the later can be as Enhancer for the food.The best conditions of extracting flavonoids are Extraction time 40min,extraction temperature 65℃,ratio of feed liquid 1:25,ultrasonic power 240 W.Each factors affecting on the yield of flavonoids is different in Orthogonal experiments,the extent of them is B＞C＞D＞A,that is,extraction temperature＞ratio of feed liquid＞ultrasonic power＞extraction time,and the contents of flavonoids which was extracted with the best conditions can be reached 0.7226 mg/g.The preliminary identification of purified extracted by the color reaction and ultraviolet and infrared spectra show that the flavonesis flavonols.The results of use experiment showed that the bamboo shoot flavonols has obvious anti-oxidation on oil and its antioxidant capacity stronger than Rutin.
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