Optimization On The Rapid Propagation System And Analysis On The Active Compound Of Aloe Vera L

Aloe has extremely high value in medical treatment,cosmetology and health care.The amount of its unique active ingredient extracted for industrial using is very large.Aloe industry has formed a large-scale,high-quality standardization and value-added high-tech industry trend.Research on different species of aloe in vitro breeding system optimization technology and its secondary metabolites system and culture of different varieties and types of aloe characteristics of the active compound in the development of aloe biotechnology industry is an important theoretical and practical significanceWe in this study draw materials from three kinds of Aloes at different time or part,then investigate their calli induction difference so as to find the best nutritive medium in each period of rapid propagation;meanwhile,we investigated the content of the active compounds.Through the research,we get the the optimization parameters of the various stages of rapid propagation and secondary metabolites system and Content of main active compound of different types of Aloe.For A.barbadensis Mill,the best time for drawing material for rapid propagation is on March and the best part is the second segment below stem apex. The best sterilization method is as follows:drawing 2～3 stem apex surrounded by leaves and washing with water for 30 minutes,70%alcohol for 10 seconds,and 0.1%corrosive sublimate for 4 plus 2 minutes.The best method for inducing shoot of 3 kinds of aloe after comparison among different medium is MS plus 1.0mg/L6-BA plus 0.1mg/L NAA plus 1g/L VC,its bud rate over 89%;For differentiation,there is obvious difference among different aloes.The most suitable medium for differentiation is:forA.barbadensis Mill,MS plus 2.5mg/L6-BA plus 0.1mg/L NAA;forA.arborescens Mill,MS plus 3.0mg/L 6-BA plus 0.1mg/L NAA and for A.Chinese Baker,MS plus 1.5mg/L 6-BA plus 0.1mg/L NAA,The differentiation factor to 5.78,5.13,6.1;For inducing roots,there is also difference of medium among different aloes:forA.barbadensis Mill,MS plus 3.0mg/L IBA;forA.arborescens Mill,MS plus 2.0mg/L NAA and MS plus 2.0mg/L IBA;forA.Chinese Baker,,MS plus 3.0mg/L NAA and MS plus 3.0mg/L IBA.All can make the chance of rooting up to 100%.Addition of charcoal and roots play important roles in the viability of aloes after implantation.For secondary metabolism, the best medium for callus induction is 20mg/L NAA plus 0.1mg/L KT;B5 medium (PH 6,glucose 2%) is a kind of suspension culture medium which is better for cell growth.For greenhouse bud and callus tissue derived from A.barbadensis Mill, A.arborescens Mill and A.Chinese Baker,,we investigated the content of anthraquinone and polysaccharide using magnesium acetate-methanol chromatometry and anthraquinone chromatometry.Our result indicates that both greenhouse buds and callus tissue from A.arborescens Mill are very suitable to extract anthraquinone,its Contents were 250.709 ug / ml and 30.434 ug / ml; A.Chinese Baker is the first option for extraction of polysaccharide,its Contents were 8751 ug / ml and 706 ug / ml.