The Phylogenetic Relation Of Trypanosome Based On 18S RRNA Gene Sequencing

The phylogenetic relation of trypanosoma sp in Hubei, guangxi, xinjiang , Zhejiang province and a strain of trypanosoma bruce were analyzed by using 18S rRNA sequence. The genome DNA was extracted from the red blood cells or blood of the infected mouse which include as isolates of trypanosoma from these different geographic areas. The gene specific primers were devised according to the pubulished 18srRNA sequence at GenBank. The target DNA segment was amplified by polymerase chain reaction, the PCR product was ligated into PGEM-Teasy vector. It was identified by PCR and sequencing. The results suggested that the length of 18S rRNA gene seven trypanosoma sp involved in this study was around 2188bp. Two phylogenetic trees was inferred based on 18S rRNA sequence of six Trypanosoma evansi strains in Hubei, xinjiang, Zhejiang, guangxi province, a trypanosoma bruce strain and the other species of trypanosoma available in GenBank. In the first tree, it was analyzed that the homologies of seven trypanosoma strains are 99.8%, 100% respectively. The homology between them and 6 of 13 foreign trypanosoma were 99%-100%, 62% between them and 7 foreign trypanosoma strains.