| All research data show that the piglets coccidiosis diarrhea has become an important pathogen piglets one, but for a long time the disease did not draw enough attention to you. To master piglets coccidiosis current prevalence and types of distribution in Zhengzhou region, Henan Province, We use saturated sugar - salt water solution floating method 16 different sizes coccidia piglets infection conducted an initial investigation, in July-November 2006. Results showed that the overall infection rate of coccidia were 7.97%, 26.48%, 5.89%, 15.14%, 3.12%, 6.67%, 0.76%, 11.11%, 33.57%, 22.35%, 38.87%, 4.55%, 11.47%, 17.96%, 5.13% and 22.87%,the average rate was 13.33%; Coccidia infection and its spore were 5.36%, 26.48%, 3.96%, 11.36%, 2.13%, 2.22%, 0%, 4.67%, 22.54%, 16. 32%, 30.12%, 1.96%, 7.98%, 10.15%, 4.06% and 18.48%, the average rate was 10.14%. In the pig farms in the survey, Coccidia infection have shown piglets positive, the highest infection rate was 38.87%, the lowest rate was 0.76%; but, and The highest infection of coccidia was 30.12%, the lowest rate was 0%. Infection day mainly concentrated in 11～20 days, the infection rate was 22.97%, followed by 21～30 days, next was 15.81%, again in 1～10 days, the infection rate was 13.92%; Coccidia infection, and spore age are mainly concentrated in 11～20 days, the infection rate was 21.08%, followed by 1～10 days, the infection rate was 12.87%, the lowest rate of 0% in the 41～60 days.Using image analysis and processing system Miaspro,we measurement oocysts, the sporangium size and oocysts perimeter, area, shape factor,etc. Light microscopy observation, separation, identification of the Zhengzhou region coccidia 2 genera, 8 kinds, types and Coccidia infection rates are as follows: Isospora suis,6.77%; Eimeria yanglingensissp,5.26%;E.porci,5.18%;E.debliecki,4.28%;E.suis,4.13%;E.perminuta,3. 98%;E.scabra,3.24%;neodebliecki, 2. 64%.Singular oocysts separation technology, such as Alternaria coccidia purebred swine oocysts, amplified by the 3-day-old piglets, the collection purebred offspring, and then amplified subculture, the amplified at the same time, the amplified subculture of ovulation capsule , and the process of oocysts spores spores rate, oocysts the morphological structure, etc., The results showed that: amplification and subculture coccidial oocysts get amplified, and its parent and reported in the literature, such as pigs spore coccidia same as the biological characteristics; However, pigs coccidia spore amplification study showed that ovulation piglets capsule law with the reported somewhat different, the time of the prepatent period and the patent period were a little longer than the reports. The new collection coccidial oocysts spore pigs, on 2.5% potassium dichromate solution, 28℃thermostat training, the first 26h of oocysts completely spores than reported in the literature of the earliest spores short time; 96h, the rate was 80%.The 30 one-day-old piglets of random was divided into five groups, each with six, with the exception of one group for the blank control, the other five groups each per head, as I once piglets were infected 1×10~4,5×10~4,2×10~5,5×10~5,1×10~6 spores of coccidial oocysts spore pigs, pigs, and other pathogenic spores coccidia study. Artificial infection observation after ovulation capsule of the piglets and clinical symptoms, Histological examination lesions and lesions, the results showed that: pigs coccidia ovulation spore sac latent period of 4 to 5 days, demonstrating a period of 18 to 23 days, 2 ovulation capsule at its peak, were PID7～8 and PID12～17. Spore pigs infected with coccidia appetite of piglets dropped, weary, Like lying, gray or white skin, growth slow, walk shaking; stool started paste, ash brown, the stench, as her illness became more serious and feces were gray and black fluid, and gradually turned into a yellow and khaki-colored, watery diarrhea; Some droppings are white or gray and white clabber. The pathological chang mainly concentrated in the midjejunum mucous ang inferjejunum mucous, We can see some inflammation, The ileum intumesce, and mucous appear hemorrhage dot; The midjejunum mucous appear hemorrhage spot; The inferjejunum intumesce, and mucous appear hemorrhage spot; The mesentery lymph node turgescence and take red, intestines turned thin, and mucous blooding; The mesentery lymph node turgescence and take red, intestines turned thin, andintestines turn thick; Under the microscope can see jejunum middle and posterior segment, ileum of villous atrophy, fusion, fracture, intestinal crypt hyperplasia, part of the intestinal epithelial cells has been exfoliated; lamina propria and submucosa a monocyte macrophages and lymphocytes infiltrating phenomenon Occasionally can see eosinophilic cell infiltration; ileal mucosa of intraepithelial can see a large number of goblet cell hyperplasia. In the middle and latter jejunum, ileum of mucosal epithelial cells, intestinal crypt middle of the parasites can be seen parasitophorous vacuole, in its internal maturity can see some dual-core shape, sprouting within the reproductive type I merozoite body, the multi-core type schizont, merozoite and circular gametophyte.The spore pigs Pathogenicity of coccidia, purebred oocysts amplification and subculture amplification showed that the infected piglets ovulation capsule dose of infection due to the differences of different.The results of this study indicate that coccidiosis piglets prevalent in Zhengzhou region, the types of infection have eight kinds, types and strengths of the major pathogenic species is coccidia spore pigs, the piglets its pathogenicity strong, capable of piglets coccidiosis typical symptoms and pathological changes, such as pigs, Health spore coccidia parasites in the developmental stages of the jejunum piglets middle and posterior segment and ileal epithelial cells. |
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