Effect Of Feeding Diets With Coriolus Versciclor Polysaccharides On The Immune Function And Physiological And Biochemical Characteristic Of Carassius Auratus

The paper studied effects of Coriolus versciclor polysaccharides (CVP) on the non-specific immune function, the ability of immune response and the physiological and biochemical characteristic in blood of Carassius auratus. The main results were summarized as follows:1. In order to study the effects of CVP on the non-specific immune function of C. auratus, the concentrations of CVP in six groups were respectively 0g/kg (group C₀), 0.25g/kg (group C_0.25), 0.5g/kg (group C_0.5), 1. 0g/kg (group C_1.0), 2.0g/kg (group C_2.0) and 4.0g/kg (group C_4.0). During the experiment, fishes of different groups were fed with appropriate feed separately and the phagocytic activity of leukocytes, lysozyme activity, Superoxide dismutase (SOD) activity and the content of complement C3, C4 in serum of C. auratus were tested at 0 day, 7^th day, 14^th day, 21^st day, 28^th day, 35^th day, 42^th day and 56^th day. The results showed that the addition of 0.25g/kg CVP in diet had no siginificant effect on the fish of phagocytic activity of leukocytes, lysozyme activity, SOD activity, the content of complement C3, C4 in serum of C. auratus (P>0.05). However, the non-specific immune function was markedly enhanced by dietary 0.5g/kg and 1.0g/kg CVP, especially, the effects of 1.0g/kg CVP were best during 14-21 days (P<0.05). When the addition was 2.0g/kg and 4.0/kg, these indexes were much lower than that in the control group at the anaphase of the experiment. And the results indicated that the non-specific immune function of C. auratus was enhanced by dietary 0.5g/kg to 1 .0g/kg CVP.2. In the test to study the effects of CVP on the ability of immune response of C. auratus, the influences were determined by the phagocytic activity of leukocytes, lysozyme activity in serum, the content of serum complement C3, C4, the red blood cell C_3b receptors rosette forming rate (RBC-C_3bRR), the red blood cell immunocomplex rosette forming rate (RBC-ICR), agglutinating antibody titer in the serum and relative percent survival (RPS) by challenging with Aeromonas hydrophila. Fishes were divided into 8 groups, and fishes in group C₀, group C_0.5, group C_1.0 and group C_2.0 were injected with physiological saline and in group I₀, group I_0.5, group I_1.0 and group I_2.0 they were all inoculated with formalin killed A. hydrophila The concentrations of CVP in group C_0.5 and group I_0.5 was 0.5g/kg, which in group C_1.0 and groupI_1.0 was 1.0g/kg, which in group C_2.0 and groupI_2.0 was 2.0g/kg, and the feed of group C₀ and group I₀ didn't contain CVP. The immune indexes were tested on the 0 day, 7^th day, 14^th day, 21^st day, 28^th day, and 35^th day of the experiment and the RPS was also recorded after challeng with A. hydrophila. The results showed that:(1) The phagocytic activity of leukocytes, lysozyme activity and the content of serum complement C3, C4 in serum of the immunized C. auratus were markedly enhanced by dietary 0.5g/kg and 1.0g/kg CVP (P<0.05). Howere, these indexes were restrained by dietary 2.0g/kg CVP at the anaphase of the experiment.(2) CVP could improve the phagocytic activity of leukocytes, lysozyme activity and the content of serum complement C3, C4 in serum of non-immunized C. auratus, and the trend is the same as the effects of CVP on immunized C. auratus. But these indexes of non-immunized C. auratus is lower than that were immunized.(3) RBC-C_3bRR of C.auratus was markedly improved and RBC-ICR was markedly debased by dietary 0.5g/kg and 1.0g/kg CVP at 21^st day and 28^th day. RBC-C_3bRR of C. auratus was debased and RBC-ICR was improved by dietary 2.0g/kg CVP, but there were no significant differences in RBC-C_3bRR and RBC-ICR among control group and other testing group (P>0.05).(4) CVP could improve agglutinating antibody titer of C. auratus. The agglutinating antibody titer of immunized C. auratus was highest by dietary 1.0g/kg CVP at 21^th day, and the highest agglutinating antibody titer was 597.3(5) CVP could improve the survival rate of C. auratus after challenged with A. hydrophila. Meantime, the survival rate and RPS of immunized C. auratus was highest by dietary 1 .0g/kg CVP, and they was respectively 85%, 57.14.3. In order to study the effects of CVP on the physiological and biochemical characteristic in blood of C. auratus, the concentrations of CVP in six groups were respectively 0g/kg (group C₀), 0.25g/kg (group C_0.25), 0.5g/kg (group C_0.5), 1.0g/kg (group C_1.0), 2.0g/kg (group C_2.0) and 4.0g/kg (group C_4.0). Some physiological parameters such as the number of red blood cell, he number of white blood cell, the content of hemoglobin, sedimentation rate of red blood cell and some biochemical parameters such as the concent of Glucose, Cholesterol, Triglycerides, Total protein, Albumin, Urea and the activity of Glutamate-oxaloacetate transaminase (GOT), the activity of Glutamate-pyruvate transaminase (GPT) and the activity of Alkaline phosphatase(ALP) in the serum were assayed at 0 day, 14^th day, 28^th day, 42^th day and 56^th day. The results showed that: When the concentrations of CVP was 0.25g/kg, there were no significant differences in the physiological parameters and biochemical parameters of C. auratus, and it had less effects on physiological function of C. auratus. 0.5g/kg¹.0g/kg CVP of dietary could markedly elevated the number of red blood cell, white blood cell, the content of hemoglobin and reduced the sedimentation rate of red blood for 14 days or 28 days. When the experiment continued for more than 42 days or 56 days, the concent of Glucose, Urea, Cholesterol, Triglycerides and the activity of GOT and GPT in the serum declined, but the concent of Total protein, Albumin and the activity of ALP in the serum increased. It indicated that 0.5g/kg-1.0g/kg CVP could improve the physiological function and metabolism intensity of C. auratus. However, when the fishes were fed with 2.0g/kg-4.0g/kg CVP for long time, the number of red blood cell, white blood cell, the content of hemoglobin, the concent of Total protein, Albumin and the activity of ALP in the serum of C. auratus declined, the sedimentation rate of red blood, the concent of Glucose, Urea, Cholesterol, Triglycerides and the activity of GOT and GPT in the serum increased, indicating weakened oxygen transportation, absorption and excretion.