| The study was mainly to probe an efficient way through tissues culture and rapid propagation of Smilax china L. The stems with axillary buds, stems and leaves were taken as explants. The factors that affect every step including basic medium, hormone and active carbon were detailed investigated through the direct as well as indirect way. As a result, the optimum mediums of every step were screened. A plant regeneration system of Smilax china L. was established in this research. The main results are below:①April was the best season to draw explants, in April, the germination rate were 93.33%,75.00% and 53.33% by stems with axillary buds, stems, and leaves respectively, the contaminated rate were 13.33%,16.67%,21.67% by stem with axillary buds, stems and leaves respectively, the browning rate were 35.00%,43.33%,51.67% by stems with axillary buds, stems, and leaves respectively.②5 minutes was good to treat the stems with axillary buds and stems by 0.1 % HgCl2. 3 minutes was good to treat leaves by 0.1 % HgCl2.③Different treatments of controlling browning: Cutting the leaves into 1.0cm2 was an appropriate way. Dark treatment for 10 days was the best way for decreasing browning rate. The browning rates were 20.00%, 26.67% and 30.00% and by stem with axillary buds, stem, and leaves respectively.④Induction of axillary buds on stems: The stems with axillary buds of young branches were taken as explants. The optimum culture medium for axillary buds induction was MS+6-BA1. 00mg/L+NAA0. 01 mg/L+IBA0. 05mg/L, the induction rate was 75.00%, the browning rate was 16. 67%.⑤Induction of callus: The stems and leaves of young branches from open air can not used for inducing callus. However, the aseptic stems and leaves in vitro can be used for inducing callus, and the optimum medium was MS+6-BA2.00mg/L+NAA0.05mg/L+2, 4-D2.00mg/L and MS+6-BA2.00mg/L+NAA0.10mg/L+2,4-D2.00mg/L respectively. The induction rates were 36.67% and 43.33% respectively.⑥Proliferating cultivation of axillary buds: The optimum culture medium of proliferating axillary buds was MS+6-BA2.00mg/L+NAA0.10mg/L. The multiplication rate was 4.30. The efficiency shoots is 2.40.⑦Proliferating and differentiating cultivation of callus: The optimum culture medium of proliferating callus was MS+6-BA2.00mg/L+NAA0.10mg/L+2,4-D3.00mg/L, Proliferating rate was 60.00%.The optimum culture medium of differentiating callus was MS+6-BA1. 00mg/L+NAA0.10mg/L, differentiating rate is 48.89%.⑧Rooting culture: The optimum culture medium for rooting was 1/4MS +NAA3.00 mg/L+AC1.00g/L.The rooting rate was 80.00%, and it was higher in culture medium which was added AC.⑨transplanting: The transplant medium in vermiculite, perlite and riversand by 3: 2: 1 is the optimal for Smilax china L. In which survival rate was 86.67%.
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