| Diospyros kaki Thunb. cv. Uenishiwase is one of Japanese persimmon varieties. Dormant buds taken from Uenishiwase persimmon trees, as in vitro explants materials, were established successfully. New shoots propagation, shoots rooting and plant regeneration were studied in different basal medium added different hormones. All of the researches in the paper could be useful for modified gene persimmon, through Agrobacterium-mediated transforming, and for fast-propagation of persimmon plants as well. Results as the following:1 Disinfected dormant buds from Uenishiwase-were established in modified MS medium supplemented with ZT 1.0 mg/L and IB A 0.1 mg/L; Micropropagation shoots were subcultured in MS(1/2N) medium added ZT 1.0 +IAA 0.1 mg/L for about 14-15 times, then shoots were transferred to MS medium added ZT 1.0 + IAA 0.1 mg/L or ZT 0.5+BA 1.0+IAA 0.1 mg/L. Shoots grew much better in MS than MS(1/2N) in shoots height and multiple value; and also there was no difference for shoots grown in MS medium adding ZT 1.0mg/L or ZT0.5+BA1.0mg/L. Mere clustering shoots grew in DKW medium than MS medium. The auxin IAA 0.1-0.2 mg/L was superior to IBA or NAA for propagation of Uenishiwase.2 Effects of basal mediums, hormones and their concentrations on shoots regeneration from leaves of Uenishiwase were studied by orthogonal design trial. The results showed that MS (1/2N) was the most optimum for the regeneration and 1/2 MS was better than MS. New shoots differentiation percentage in the medium containing 4.0 mg/L ZT was much higher than 2.0 mg/L and 1.0 mg /L ZT. Differentiated shoots and average shoots per explants were dramatically decreased in the 0.2 mg/L IAA. In the orthogonal trial the best result was achieved in MS (1/2N) medium containing 4.0 mg/LZT and 1.0 mg/L IAA, in which shoot percentage and average shoots per explants were 86% and 2.21 respectively. Green, loose and nodular callus was differentiated from leaves of Uenishiwase in MS(1/2N) medium supplemented with 0.1-0.2 mg/L NAA and 1.0 mg/L ZT; and adventitious shoots were induced on these callus in MS(1/2N) medium supplemented with ZT 2.0 +IAA0.1 mg/L, differentiated percentage was about 75% and average adventitious shoots per calluswas 2.5. Callus of leave induced by 2,4-D could not differentiate shoots.Callus was induced on leaves of Uenishiwase by orthogonal design trial. One hundred percent of leaves differentiated callus in MS(1/2N) medium added TDZ 1.0 + ZT 1.0+ IAA 0.1 mg/L; ninety-five percent callus differentiated adventitious shoots, and average shoots was about 1.95. Callus induced on leaves of Uenishiwase in TDZ 1.0 mg/L was transferred to MS(1/2N) medium added ZT 2.0 + IAA 0.1 mg/L, then number of differentiated shoots were about 9.5, differentiated shoots percentage was 100%.New shoots were differentiated from roots of Uenishiwase shoots much better in MS(1/2N) medium than MS; ZT 2.0 mg/L added in medium was superior to ZT 1.0 + BA 1.0 mg/L. One hundred percent of roots in 40~50mm length could differentiate new shoots, and number of shoots was 3.84. Linear regression relation was distinctive between roots length and no. of new shoots from roots; regression equation was Y=0.03671+0.0896X. And added 0.1 mg/LIAA in MS(1/2N) medium could increase number of new shoots from roots distinctively.3 Roots from Uenishiwase shoots formed by means of dipping auxin solution or adding auxins into 1/2MS(1/2N) medium. IBA was more effective than IAA: after shoots in 20~25mm length were dipped into 250 mg/L IBA solution for 15 min, 66.67 percent rooted, and number of roots was 1.7; 64.58 percent shoots rooted, cultured in medium containing IBA 1.0 mg/L, number of roots was 1.6. Shoots cultured hi MS medium containing BA1.0+ZT0.5mg/L showed higher ability for rooting than containing ZT 1.0mm/L. Regeneration shoots from roots were easier for rooting than regeneration shoots from leaves, subculture shoots; high rooting ability in sequence was regeneration shoots from roots, regeneration shoots from leave, subculture shoots containing BA+ZT, subculture shoots...
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