Study On The Saponins Of American Ginseng Berry Juice

This study researched on the separation and identification of saponins from American ginseng berry juice (AGBJ). The selected macroporous resins , ordinary and middle-pressure chromatography, sephadex LH-20 and HSCCC techniques were used on the isolation of extract after the decolorization . The research laid a theoretical and methodological foundation for effective exploitation and sustainable utilization of AGBJ. The results are as follows:1. Ginsenoside Re and Rg1 were separated from AGBJ by HSCCC for the first time with Acetoacetate-n-butanol-water (4:1:7) as the solvent system. The flow-rate is 2.0 ml/min and rotate-rate is 810 r/min. The retention time of Re and Rg1 were 140～165 and 195～228min,respectively. The HPLC chromatogram with UV at 203nm showed that the purity of ginsenoside Re and Rg1 were above 98.55% and 98.42%.2. The saponins in AGBJ were isolated and prepared by macroporous resins and chromatography under ordinary and middle-pressure. The extracts within 35% and 50% ethanol part ofmacroporous resins were isolated by ordinary and middle-pressure chromatography. Seven constituents were gained and identified as 20(s)-ginsenoside Rh2, ginsenoside Re, ginsenoside Rc, ginseno-side Rb2, ginsenoside Rd, ginsenoside Rb1, and ginsenoside Rg1. by physical-chemical and organic spectroscopy.3. The effects of MgO and activated carbon on decolorization of AGBJ were also studied by changing amount of MgO, heating temperature and heating time. We concluded that MgO was suitable for the decolorization of AGBJ and MgO should be 3 times of AGBJ extract dry weight. The heating temperature and heating time had little influence on loss of saponins.The innovations of this study:1. It is the first time that middle-pressure chromatography was used to research constituents of AGBJ and the gradient elution mode of dichloromethane-methanol by middle-pressure chromatography was set up.2. High-Speed Counter-Current Chromatography was used first time to separate and prepare ginsenoside Re and Rg1 in AGBJ.