Study On The Callus Induction And Regeneration Of Bambusa Emeiensis 'Viridiflavus'

Bambusa emeiensis'viridiflavus'is a graceful bamboo for virescence and view. This research focused on the callus induction and organ regeneration of this plant, and recorded the whole process of callus induction, proliferation and differentiation by the methods of plant cytology.The results were:①The best basic condition for callus culture were MS medium with sucrose 30g·L^-1, buds from germfree plantlet as explants, and Vitamin C 100mg·L^-1 as browning-preventative reagent.②The suitable medium for callus induction were MS+2,4-D5mg·L^-1+BA1mg·L^-1. According to the analysis, we made a conclusion on the origin of callus: callus tissue came from the active cells of primary meristem at the periphery of procambium which derived from apical meristem.After callus induction we could get three different types of callus:Type I: This type of callus was light yellow or light kelly, had a granulating surface and a hard texture. We saw the close-knit, round or ellipse cell from microstructure. The cells had thin cell walls, big nucleolus and grumous cytoplasm. And there was abundance inclusion between the cells.Type II: This type of callus was gray or light brown, and always had a soft touch. We could see the incompact arrange of the cells from microstructure observation. The cells were round, ellipse or strip, always had a little nucleolus declined to one side of the cell. The cells of callus type II had a big vacuole and rareness cytoplasm. Between the cells there was rareness inclusion.Type III: This type of callus was often white or translucent, very soft and easy to separate. They always had a high hydrated rate. We could see that this type of callus was formed by ashine grape-look cell groups under anatomic microscope.③From the paper, the feasible medium for callus proliferation of type I were MS+2, 4-D3mg·L^-1+BA0.2mg·L^-1+NAA0.5mg·L^-1.Proliferated callus had thin cell walls, grumous cytoplasm, abundance organelles and a great deal of mitochondria and endoplasmic reticulum. And we also could see some little dispersive vacuoles in the cell according sub-microstructure observation.④The optimism medium for differentiation were MS+6-BA5mg·L^-1+NAA0.2mg·L^-1.⑤According to the data, the best rooting medium for plantlet generated from callus type I were MS+6-BA0.5mg·L^-1+NAA1mg·L^-1+IBA0.1mg·L^-1.