The Establishment And Optimization Of Bambusa Ventricosa In Callus Regeneration System

It is difficult and rare to establish the regeneration system of Bamboo plants by inducingcallus in the current research. It is also a hot topic.This research focused on the callus induction and regeneration.The results were:1, The suitable medium for callus induction was:MS+6mg/L2,4-D+0.5mg/LNAA+0.001mg/L TDZ.After callus induction, two different types of callus were abtained:Type I:It was the best callus for proliferation, which was yellowish-white, hard texture,loose granular. Each compact callus granular was low moisture and bright color.Type II:This type of callus was bubble-shaped, white or translucent. They were highmoisture content, viscous.2,The callus could be induced by terminal bud(1.5cm long),node with buds, leaf.Theterminal bud was the best explant in the rate of callus induction. The callus induction rate was63.5%, and the node with buds followed.3,The best medium for callus proliferation of type I was: MS+5mg/L2,4-D+0.5mg/L6-BA+1mg/L NAA+100mg/L Vc.Three types were obtained after proliferation:I-1,light white,hard texture, and granular;I-2, bubble-shaped, white or translucent;I-3,white with black callus,high moisture.4, The optimism medium for differentiation of embryogenic callus was:MS+3mg/L6-BA+0.5mg/L NAA.5,The rooting formula was followed by that of Bambusa emeiensis 'Viridiflavus':MS+0.5mg/L6-BA+1mg/L NAA+0.1mg/L IBA,and the rooting rate was25%.6,The best concentration of PPT was40mg/L and Hyg was50mg/L by testing theresistance of callus of type I-1.