Genetic Diversity Among Populations Of Lilium Regale

Genetic diversity and genetic differentiation among ten populations of Lilium regale,an endemic species in China,were analyzed using Inter Simple Sequence Repeat(ISSR)molecular technique.The main results were as follows:(1)There were 179 polymorphic loci among 180 ISSR loci of 10 primes selected from 165 ISSR primers.The total percentage of polymorphic loci(PPL)was 99.44%.(2)On the species level,the observed number of alleles(Na)and effective number of alleles(Ne)of Lilium regale was 1.9944 and 1.2916,respectively. Nei's gene diversity(h)and Shannon's information index(I)of Lilium regale at species level was 0.2029 and 0.3398.These data indicated that the genetic diversity at species level of Lilium regale was relatively high.(3)On the population level,the population of Taoping Cun had the highest percentage of polymorphic loci(PPL=81.11%),while the population of Guangming Xiang was the lowest(PPL=63.56%).The population of Feihong Yinpo had the highest(Ne=1.3211),and the population of Guangming Xiang was the lowest with the effective number of alleles(Ne=1.2011).The population of Feihong Yinpo had the highest Nei's gene diversity(h= 0.2018),while the population of Guangming Xiang was the lowest(h= 0.1397).The Shannon diversity index of Feihong Yinpo population was the highest(I=0.3180),while the Shannon diversity index of Guangming Xiang population was the lowest(1=0.2396).(4)As analyzed by POPGENE,the genetic differentiation coefficient(Gst) among populations was 0.1894.The Analysis of molecular variance (AMOVA)demonstrated that the genetic differentiation coefficient among populations 0.1934(PHIst=0.1934).And the genetic differentiation coefficient among populations((Hsp-Hpop)/Hsp)calculated by the Shannon diversity index was 0.2038.These data demonstrated that the genetic variation of within-population accounts for the most of genetic differentiation among Lilium regale populations.(5)The dendrograms of genetic relationships among populations were constructed based on Nei's(1978)unbiased genetic distance by means of UPGMA showed that the 10 natural Lilium regale populations could be divided into 3 big groups,the population of Guangming Xiang was included in group 1,the populations of Yangmao Ping,Feihong Yangpo and Feihong Yinpo populations were in group 2,population Shida Guan,population Taoping Cun,population Seer Gu,population Huangyan Dong,population Xuecheng Zhen and population Tazi Shan were clustered into group 3.(6)The results of correlation analysis(r=0.0936,p＞0.05),showed that there was no significant correlation existed between geographical distance and Nei's unbiased genetic distance of the 10 Lilium regale populations.However,The results of Mantel Test(r=0.5259,p＜0.05)showed that there was significant correlation between Nei's unbiased genetic distance of the Lilium regale populations and the distance of elevation.(7)For phenotype diversity analysis,the 10 populations were divided into 5 groups according to Mahalanbis Distance by means of UPGMA.The population of Guangming Xiang was included in group 1,the population of Feihong Yangpo was included in group 2,the populations of Taoping Cun and Tazi Shan were included in group 3,the populations of Yangmao Ping, Huangyan Dong were clustered into group 4,population Feihong Yinpo, population Shida Guan,populations Seer Gu and population Xuecheng Zhen were clustered into group 5.(8)The results of correlation analysis(r=0.6800,p＜0.05),showed that there was significant correlation existed between Mahalanbis Distance and Nei's unbiased genetic distance of the 10 Lilium regale populations by Mantel Test of TFPGA.At the same time,significant correlation was found between geographical distance and Mahalanbis distance of the 10 Lilium regale populations.