Genetic Analysis And SSR Mapping Of Stripe Rust Resistance Of Triticum Aestivum-Hayaldia Villosa Translocation Lines

Stripe rust of wheat is one of most serious disease in the world. The research and production practice,both at home and abroad, has proved that the most economical, effective, feasible and environment friendly nuclear methods to control this disease is. planting resistant cultivars. But one cultivar breakdown its resistance in several years .So, the key.to solve the problem of Wheat varieties resistant to stripe rust loss is to find and use of high-quality source of new wheat varieties.In the research, resistance to stripe rust of three Triticum aestivum- Hayaldia Villosa translocation lines and the genetic mechanism of their resistance to stripe rust were analyse systematically,then ,SSR markers of one stripe rust resistence gene in translocation line V9128-3 was investigated.The results are as follows:1.Using the current epidemic yellow rust races CYR29,CYR30,CYR31,CYR32 and Su-4,Su-11,Su-14 inoculated to three Triticum aestivum-Haynaldia villosa translocation lines V9128-1,V9128-3 and V3 for resistance evaluation. The results showed the three Triticum aestivum-Haynaldia villosa translocation lines possess high-blooded resistance.to the current epidemic yellow rust races.2. It showed by inheritance analysis of three translocation lines resistance to stripe rust that:(1)Single dominant gene confer resistance to CYR30,CYR32-6 and Su-4, one dominant gene and one recessive gene to CYR31 in V9128-1.(2) Single dominant gene confer resistance to each CYR30,CYR32-6, CYR31 and Su-4 in V9128-3.(3) Two complementary dominant genes confer resistance to both CYR32-6 and Su-4, one dominant gene and one recessive gene confer independently or single dominant gene confer resistance to CYR31 inV3.(4) V9128-1and V9128-3 maybe contain same or tightly linked genes confer resistance to CYR32-6, CYR31 and Su-4.(5) V9128-1and V3 contain different genes confer resistance to CYR30,CYR32-6 and Su-4, maybe contain at least one same or tightly linked gene confer resistance to CYR31.(6) V9128-3 and V3 contain different genes confer resistance to CYR30,CYR32-6 and Su-4, maybe contain same or tightly genes confer resistance to CYR31. 3. The gene in translocation line V9128-3 which confer resistance to Su-4 was developed SSR markers, two markers Xgwm356 and Xwmc658 from 219 SSR primer combinations we had used were found linked to YrHV(temporarily designated).YrHV was 8.5cM from Xgwm356,and 5.6cM from Xwmc658.Therefore, YrHV was located in the chromosome 2AL.Moreover, a portion of BC1F1 individuals and F3 lines were used to validate that the two sites were linked to YrHV.This study revealed genetic mechanism of translocation lines resistance to stripe rust and obtain moleculer markers. It provided theory values for utilizing these resistance genes scientifically.Then ,we can use these markers for marker-assisted selection for the breeding new wheat cultivars.