Study On The Occurency Of Three Kinds Of Virus On Lilium From Qin-Ba Mountains And Lily Cultivars Under The Nursery Field

With 6 Llilium species(L.regale, L. tigrinum, L.brownii, L. leichtlinii var. Maximowiczii, L. pumilum and L. leucanthum) collected from Qin-ba Mountains, and 17 lily cultivars of different hybrids(Stargazer, Siberia, Bernini, Soldera, Kiev, Tiber, Sorbonne, Corl`dool, Manissa, Boliana, Prato, Corvara, Lombardia, Medusa, ConcaD`or, Yelloween, Longiflorum×Asian) from different fields,the severity of virus endangerment was investigated based on the vision count and three kinds of virus (Cucumber mosaic virus, Lily mottle virus, Lily syptomless virus) were identified by the RT-PCR with the leaves samples collected in different growth periods under the nursery field. The research aims was to make sure if the original Lilium species and the lily cultivars were infected or not by the virus in the local distributed areas, and to identify which virus types were occurred.Then make sure if the virus occurred when the original Lilium species and the lily cultivars planted under the nursery field and identify which virus types were occurred in the lilium plants .The resistance to virus of the wild lilium species was preliminary evaluated for being as materials in the coming breeding program.It was resulted that:1. In comparison with different observations and identification of virus occurrence under different locations of lilium plants, the wild Lilium species of L. regale, L. brownii, L. leichtlinii var. Maximowiczii, L. pumilum and L. leucanthum were not infected by the virus in the wildly grown plants in the original areas,only L×A(Longiflorum×Asian) of the lily cultivars was infected by LMoV in the original area Yunnan in all the cultivars.2. The lilium species are easily infected by the virus when they grown in the nursery field without protection measures.It was observed for the symptoms of mosic and mottle with special virus symptoms of the leaves in 5 lilium species under the cultivation in the nursery field ,but no virus symptoms was observed in L. regale. In all of the lily cultivars, the virus occurred only on the L×A cultivar during the growth period, and it was mildly transmitted, the incidence of virus disease spread from 66.5% to 71.3%.Otherwise, the Siberia cultivar from Fujian was seriously observed for the special virus symptoms of mosic and mottle .3.Through the RT-PCR analysis, it was proved that there was no virus infection in L. regale, that L. tigrinum was infected by LMoV and that L. brownii, L. leichtlinii var. Maximowiczii, L. pumilum and L. leucanthum were multiply infected by CMV and LMoV. It can be concluded that there were different resistances among the different lilium species; that L. regale was immune to virus; L. leichtlinii var. Maximowiczii and L. leucanthum were medially susceptible, but that L. tigrinum, L. brownii and L. pumilum were highly susceptible.It was proved that only LMoV occurred on the L×A cultivar during the growth period. The lily cultivar of Siberia from Fujian was multiply infected by three of the CMV, LMoV and LSV viruses.