Studies On Prescription Sieving And Preparation Technology Of The Traditional Chinese Medicine Granules Against Calf Diarrhea Caused By Bacteria

Calf diarrhea caused by bacteria threaten husbandry greatly,and the abuse of antibacterials makes its cure more difficult,traditional Chinese medicine plays an important role for its special superiority.13 traditional Chinese medicines were chosen in this topic,they were:Fructus mume,Fructus forsythiae,Radix scutellariae,Flos lonicerae,Radix adenophorae,Pericarpium granati,Rhizome picrorhizae,Radix sanguisorbae,Radix et rhizome glycyrrhizae,Fructus chebulae,Radix et rhizoma salviae miltiorrhizae,Cortex phellodendri amurensis and Radix sophorae flavescentis, which were usually used to resist bacteria.Bacteriostasis experiments in vitro were done to test the effects of those traditional Chinese medicines to resist the main pathogenic bacteria,which were Escherichia coli O₈,Clostridieum welchii type B,Clostridieum welchii type C and Salmonella typhimurium,by Scrip method.Physic liquors were extracted by water or alcohol.The results showed that alcohol extracted Rhizome picrorhizae and Fructus mume both had significant inhibitory effects to the four main pathogenic bacteria.Chose Atractylodes macrocephala, Zingiberis rhizoma,Costustroot and Radix et rhizome glycyrrhizae to match with them,based on the Chinese medical science theory:cure both appearance and substance and determine the treatment based on differentiation of symptoms and signs,so as to complement each other and enhance pharmacodynamic actions.According to the clinical use and stability of preparation,drug granules were choosen as their form of medication.Extraction flow-sheet was:Rhizome picrorhizae and Fructus mume were extracted by alcohol,the optimum extraction condition was investigated with orthogonal design method by studying the content of berberine and extracta sicca;as to volatile oil contained in Atractylodes macrocephala,Zingiberis rhizoma and Costustroot,used distillation with water method to extract,and wrapped it up withβ-cyclodextrin(β-CD) to stable the preparation and ensure the therapeutic effect.Radix et rhizome glycyrrhizae could promote the dissolution of volatile oil,so it was extracted with those three medicines.The optimum extraction condition was determined by observing the imbitition amoun,soaking time,extracting time and crashing degree, based on the dissolution ratio of volatile oil.The optimum extraction condition was investigated with orthogonai design method by determing the yiels of clathratio and utilization ratio of volatile oil;there were water-solubility effective ingredient in the physic liquor of Atractylodes macrocephala,Zingiberis rhizoma,Costustroot and Radix et rhizome glycyrrhizae after extracting volatile oil,the orthogonal design method was used again by determining the content of glycyrrhizic acid and extracta sicca.Decided granulate forming technology by observing the choosen and usage amount of adjuvant and ambient humidity.The result was:Rhizome picrorhizae and Fructus mume were extracted three times by 8 times 60%alcohol,1 hour per time; Atractylodes macrocephala,Zingiberis rhizoma,Costustroot and Radix et rhizome glycyrrhizae were crushed into coarse powder,no soak,extracted 6 hours with 8 times water;clathration technology of volatile oil was:proportion ofβ-CD and volatile oil was 4:1,and that ofβ-CD and water was 1:8 clathratio for 30min under 40℃;remaining physic liquor was condensed into 0.85g·mL^-1,centrifugated 15min by 3000r·min^-1 and precipitated by adding 12 times 80%alcohol, and standing for 16h.Alcohol extracted physic liquor of Rhizome picrorhizae and Fructus mume and remaining physic liquor of Atractylodes macrocephala et aL after extracting volatile oil affiliated and decompression condensed into dry mastic,then mixed with clathrate,added partes aequales dextrine,took 95%alcohol as wetting agent,granulate on the ambient humidity below 70%.Ultraviolet spectrophotometry was used to measure the content of berberine hydrochloride in the granula.Indexes such as granularity,moisture capacity and dissolve ability were evaluated, which were consistented with drug granules regulations of edited in 2005.Accelerated stability test results showed that charactors,qualitation,granularity and contents of main effective ingredient of granula according to technology above had no change in six months.Studies above had determined the prescription and prepare technology of traditional Chinese medicine to cure calf diarrhea caused by bacteria,set groundwork for the further studies,such as pharmacodynamics,pharmacokinetics,toxicology and mechanism of action and so on.