Study On Key Techniques Of Tubers Production Of Zantedeschia Hybrida

Colorful calla lily (Zantedeschia hybrida) is a famous flower, widely cultivated through out the world. In recent years, our country spent a great deal of foreign exchange to import tubers, the cultivated area extend rapidly, However the local concerning the tubers production technique research of colorful calla lily currently is not much, so carry on a research, build up an efficiently colorful calla lily tubers production technique, this has very important and realistic meaning to reducing the foreign exchange expenditure and increasing the flower agriculture income.The material of the following studies is colorful calla lily (Zantedeschia hybrida), belongs to the family Araceae and the genus Zantedeschia. Key techniques of rapid propagation in vitro, tubers storage, dormancy release and florescence promoted were investigated. In this study, Colorful calla lily 'Black magic' were used as experiment material. In order to find a good treatment to remove pollutants from explants in the initial culture. Next experiment was to induce adventitious bud, and to find what influence on proliferation of adventitious bud under the treatments of different types and concentrations hormones. After that, different sugar content were investigated to see which treatment was good for adventitious bud to rooting and growing. Then we studied different treatments of toughening plantlets and different transplanting time which influencing on the rate of survival plantlets. Contrasting the different disinfectant, different storage temperature, different size tuber, studying the efficient treatment of reducing rot tubers which appears in the process in the storage. Studied these treatments of different size tubers, different storage temperature and different GA3Content, in order to find efficient way of dormancy release. Studied different storage temperature and different GA₃ content which influencing florescence promoted on different size tubers .The results showed:1. decrease the pollute rate in initial tissue culture: Using 0.1 Hgcl₂ pretreated explants different time. The results showed: 12min is the best time for tuber bud which growing one year,the surviving explants reached 43.3%.16min is the best time for tuber bud which growing two or three year,the survival explants is about 33.3%.2. induce and proliferation adventitious buds: The best treatment of induce and proliferation adventitious buds was BA2.0mg/L+ GA0.3mg/L +MS medium. After that, not only the proliferation multiple of adventitious buds is about 5.3,but also the adventitious buds growing strong with fresh green and good tenacity.3. Growing and rooting of plantlets: 1/2MS medium + IBA0.5mg/L+ Cane sugar 10g/L was the most useful treatment to plantlet's growing and rooting. The average number of rooting is 3.2, the plantlets and roots growing strong with right length.4. transplanting of plantlet: Toughening plantlets 3d outdoors was the most useful treatment to plantlet's transplanting, After transplanting 20d, the survival rate of plantlets was 100%, After transplanting 80d, the survival rate of plantlets was 88%. In this experiment, March 10 was the best transplanting time, After transplanting 20d,the survival rate of plantlets was 100%;with changing of time, because the environment temperature heats up, the survival rate of plantlets was falling gradually, transplanting on July 25, the survival rate of plantlets was falling to 34%.5. Decrease the percent of rot tubers in the storage: Antisepsis of tuber with 0.1% potassium permanganate or streptomycin single can decrease the rot rate, dipping in "0.1% potassium permanganate + streptomycin (15min)" was the most useful treatment to tuber's antisepsis, After storage 50d, the rot rate of one year tubers decrease 71% than CK. Experimentation show low temperature is good at reducing rot rate of tubers; in addition, the rot rate of small tubers were more than big tubers.6.dormancy release and burgeon promoted: High temperature or GA₃ was good at dormancy release and burgeon promoted for Zantedeschia hybrida tubers, high "temperature(22-25℃)+ GA3 (300 mg/kg, 15min)" was the most useful treatment to tuber's dormancy release, after 60d,the burgeon rate was 53.3%, after 80d,the burgeon rate was 93.3%. The experimentation showed in addition: the tubers which starch content reducing more burgeon earlier. 7.florescence promoted:"High temperature(22-25℃)+ GA₃ tubers (200 mg/ kg,10min)" was the most useful treatment to one year tuber's florescence promoted, the florescence rate was 36.7%."high temperature(22-25℃)+ GA₃ (300 mg/kg,10min)" was the most useful treatment to two and three years tuber's florescence promoted, the florescence rate was 66.7% and 83.3%. After treating, not only the florescence rate was high, but also the plants growing well with beautiful appearance.