| Catharanthus roseus (Linn.) G Don is a kind of perennial herbaceous plant and subshrub of the catharanthus in Aocynaceae. Due to strong adaptability and year-round abloom, it can be used in many fields, such as in medicine and in landscape architecture. There is not a perfect tissue culture technique in transformation gene and other facets; therefore, there are many economic, zoology and society benefit to study the tissue culture technique of Catharanthus roseus.The techniques of test-tube seedlings tissue culture rapid propagation in seeds of Catharanthus roseus from Hai-nan Base were studied in the experiment. The optimal culture medium and conditions for plantlet regeneration had been selected under the variant phytohormones combinations and culture conditions. Moreover, the effects of various factors on Catharanthus roseus differentiation, growth and rooting culture were discussed in the study.The results are listed as followed:1. The best method to sterilize the seeds of catharanthus roseus was marinated by suds for 30 min, washed by water for 36 h, marinated by 0.1% HgCl2 for 10 to 15 min on the Situation of Bacterium Free, and then washed by asepsis water for 4 to 6 times; subsequently, marinated by 10 % NaClO for 5 to 10 min, washed by asepsis water for 4 to 6 times, marinated by 70 % CH3CH2OH for 60 s, and then washed by asepsis water again for 4 to 6 times.2. When setting catharanthus roseus laminae, petiole, hypocotyl as explants, the effects of different kinds of homogeneity and concentrations of growth adjustive matter on inducing callus were different.3. In the combination of 6-BA and NAA, the best culture medium to induce callus was MS+6-BA 3.0 mg·L-1+NAA 0.5 mg·L-1+sugar 30 g·L-1+agar 7g·L-1; the callus induction rate reached to 95.3 %refering to laminae. The best culture medium to induce callus was MS+6-BA 3.0 mg·L-1+NAA 1.0 mg·L-1 sugar 30g·L-1+agar 7 g·L-1; the callus induction rate was to 86.9 % refering to petiole. The best culture medium to induce callus was MS+6-BA 3.0 mg·L-1+NAA 0.5 mg·L-1+sugar 30g·L-1+agar 7g·L-1 and MS+6-BA 3.0 mg·L-1+NAA 1.0 mg·L-1+sugar 30 g·L-1+agar 7 g·L-1; the callus induction rate reached to 88.6% and 89.0% repectively, refering to hypocotyl.4. No matter what used laminae, petiole or hypocotyls of catharanthus roseus.as explant, callus could not be induced with KT.5. The callus which was induced from young leaves could be used as rapid propagation materials. The optimum medium for buds multiplication was MS+6-BA 2.0 mg·L-1 (2.5 mg·L-1)+IBA 0.2 mg·L-1+GA31.0 mg·L-1+sugar 30 g·L-1+agar 7 g·L-1; the optimum medium for rooting was 1/4 MS+IBA 1.5 mg·L-1+NAA 0.05 mg·L-1+carbon 0.5 mg·L-1+sugar 20 g·L-1+agar 7g·L-1.
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