Study On Tissue Culture And Cutting Propagation Of Catharanthus Roseus (L.) G. Don

The seeds and plants were taken as explants to study sterile bud proliferation, callus induction and bud differentiation, root induction in vitro and cuttings rooting of Catharanthus roseus (L.) G. Don. The paper was mainly researched the following contents: effect of0.1%HgCl2and10%NaClO on seeds sterilization; effect of basic medium on sterile bud proliferation and rooting; effect of different combinations and concentration of plant growth regulators on sterile bud proliferation, callus induction and bud differentiation, and cuttings rooting; effect of sampling position on callus induction and bud differentiation, and cuttings rooting; effect of different hormones with various concentration in callus-inducing medium on callus bud differentiation; effect of different transplantation matrix on tissue cultured seedings; effects of different processing times of hormones on cuttings rooting.The main results are as follows:1. When using0.1%HgCl2and10%NaClO to sterilize Catharanthus roseus seeds with different sterilizing times, the10%NaClO was better than0.1%HgCl2, and10min was the optimum time. The optimum culture medium of sterile bud multiplication in this study was MS+6-BA3.0mg/L+NAA0.2mg/L+TDZ0.02mg/L2. The1/3leaf+petiole was the best in the three sampling positions for callus induction. The culture medium MS+2,4-D1.0mg/L+TDZ0.02mg/L were beneficial to induce callus in this study. The sampling positions and culture mediums of callus induction influenced callus bud differentiation. The callus which induced by1/3leaf+petiole on the culture medium MS+2,4-D0.5mg/L+KT0.5mg/L was better than the other callus for bud differentiation. In this study, the optimum culture medium of callus bud differentiation was MS+NAA0.2mg/L+6-BA2.0mg/L+KT2.5mg/L.3. Adding the right amount of activated carbon to the culture medium was conducive to root induction of Catharanthus roseus tissue culture. The culture medium1/2MS+ABT-61.0mg/L+PP3330.5mg/L+activated carbon600mg/L was the best in the root induction treatments. The peat+perlite+garden soil(1:1:1) was better than the other transplantation matrix for Catharanthus roseus tissue culture seedings transplant.4. Soaking the central position of branch in hormone solution ABT-650mg/L+NAA15mg/L for9h was conducive to Catharanthus roseus cutting propagation in this study.