The Polymorphism Analysis Of Sheep BMP15 Gene And BMPR-IB Gene

Abstract BMP15 and BMPR-IB as candidate genes on the fecundity,The polymorphism of BMP15 gene and BMPR-IB gene were detected in four sheep breeds mongolian sheep,hu lun bei er sheep,Inner mongolian fine wool sheep and china merino( ke er qin type). in order to find a molecular genetic marker which has significance in mongolian sheep breeding.(1) the primer was designed by BMP15 exon1 sequence in the GenBank, adopted PCR technique to magnify the sequence and analysis the polymorphism by PCR-SSCP of BMP15 gene in different four breeds of sheep.The results were as follows:The polymorphisms of PCR-SSCP existed in the four ewe brains,and this polymorphism show three gene types in every one brain.AA,A+,++ type;The mutation detected did not affect significantly the prolificacy of ewes.(2) the primer was designed by BMPR-IB sequence in the GenBank, adopted PCR technique to magnify the sequence and analysis the polymorphism by PCR-RFLP of BMPR-IB gene in different breeds of sheep. The results in dicated that there was a same mutation (A746G) of BMPR-IB gene in china Merino as that in Booroola Merino ewes. That Mutation did not exist in mongolian sheep,hu lun bei er sheep,Inner mongolian fine wool sheep.