Analysis On Single Nucleotide Polymorphisms Of BMP15 BMPR-IB And GDF9 Gene As Candidate Genes For Fecundity In Mongolian Sheep Breeds

This experiment with local varieties of Inner Mongolia sheep for its study object, and a double cade strains of sheep lambing strains plays an important role in the fertility of the BMP15, GDF9, BMPR - IB three genetic polymorphism of seven sites by comparison test and analysis, and they were in different strains of polymorphism sheep. By the analysis of PCR amplification and partial sequence analysis and PCR - SSCP polymorphism of PCR analysis, use polymorphic RFLP - DNAStar software to detect the polymorphic loci sequencing sequences homology analysis. Research results show that: (1) to BMP15 gene polymorphism of five sites, Mongolia sheep two strains were detected and Belclare Cambridge and sheep of the same gene mutations, B1 BMP15 28-30 nucleotide bases, cause coding region 10 amino acid residue leucine missing, B2 for point mutations without polymorphism, BMP15 gene loci in testing, B4 two strains were detected in the six-yard box scramble coils, , bases the sheep BMP15 gene mutations in the loss occurred, FecB mutation has not been detected. FecB for testing, although have found mutations, but for some of the low incidence, group in low-alcohol polymorphism. 2 for GDF9 gene polymorphism of G8 sites, get in a single cade strain detected two different genotypes, namely GDF9 genes in the area in the double 1184bp explicitly convert, and T C - led to a sheep GDF9 gene mutations. 3 for I BMPR - by PCR amplification gene sequences and the part of PCR analysis, polymorphism RFLP - Mongolia sheep with lambing double lines in this site are lambing strains without such mutations.