| The purpose of this study was the conservation of Matou goat by use of thesuperovulation,embryo cryopreservation and frozen embryo transfer techniques. Thesuperovulation, embryo cryopreservation, frozen embryo transfer were studied in thisexperiment.To improve the reproductive performance of the excellent female Matou goat, thefactors affecting superovulation, such as treatment methods, mating methods, litter sizes,body condition, repeated superovulation and gene polymorphism were firstly studied inthis experiment. The results indicated that: (1)The number of recovered embryos per goat(15.63vs12.75), the number of useful embryos per goat (14.75vs11.25) and the useful rateof embryos (94.40％vs88.24％) were significantly improved when superovulation by useof LHRH-A3 and P4, compared with the basic superovulation method of CIDR+FSH+PG;(2)The number of recovered embryos per goat, the number of useful embryos per goatand the useful rate of embryos were significantly higher than that of mating every 8 hours(male:female=1:2) or mating every 12 hours (male:female=1:1), when mating every 8hours (male:female=1:1) or artificial insemination every 8 hours (P＜0.05); (3)The numberof recovered embryos per goat (16.83vs14.17) and the number of useful embryos pergoat (15.83vs13.33) of the donors with four feta was significantly higher than the donorswith less than two feta at previous lambing (P＜0.05); (4)The number of recoveredembryos per goat, the number of useful embryos per goat and the useful rate of embryosof the donors with medium body condition were significantly higher than the donors withsuperior or inferior body condition (P＜0.05); (5)The number of recovered embryos pergoat (15.17vs15.67), the number of useful embryos per goat (14.17vs14.83) and theuseful rate of embryos (93.41％vs94.68％) had no significant difference between therepeated superovulation and the first time superovulation (P＞0.05); (6)The differentgenotype had the same effect on the number of recovered embryos per goat and thenumber of useful embryos per goat for Matou goat, it indicated that GG＞AG＞AA, butthere was no significant difference (P＞0.05).To explore the stable and reliable method of embryo cryopreservation and improvethe cryopreservation effect, the factors affecting the development effect of the frozenembryos, such as freezing methods, thawing temperature and thawing methods weresecondly studied in this experiment. The results indicated that: (1)The normoplasia embryos rate (84.09％vs81.08％) and the developed blastocyst rate (63.51％vs60.00％)had no significant difference between the straw vitrification freezing and OPSvitrification freezing(P＞0.05), while, straw vitrification freezing was better than OPSvitrification freezing; (2)The normoplasia embryos rate (83.78％vs81.48％) and thedeveloped blastocyst rate (64.52％vs59.09％) had no significant difference on thawing in25℃and 37℃water bath, for embryos freezed by straw vitrification (P＞0.05), while,37℃was better than 25℃; (3)The normoplasia embryos rate (87.50％vs83.78％) and thedeveloped blastocyst rate (66.67％vs64.52％) had no significant difference on thawing byuse of one-step and two-step, for embryos freezed by straw vitrification (P＞0.05).To further check the effect of embryo cryopreservation and the feasibility of theconservation of Matou goat by establishing frozen embryos bank, the synchronization ofestrus and frozen embryos transfer of Matou goat was finally studied in this experiment.The results indicated that: (1)The rate of synchronization of estrus by use of CIDR was85.71％, by use of progesterone sponge plug was 78.57%, the rate of synchronization ofestrus had no significant difference between them, when chosen healthy, with normaloestrous cycle, local Banjiao goat as recipients (P＞0.05); (2)The pregnancy rate ofmultiparity recipients was 46.67％, the pregnancy rate of virginal recipients was 37.50％,the pregnancy rate had no significant difference between them, when transferred Matougoat superovulation embryos after straw vitrification frozen-thawing, but the pregnancyrate of multiparity recipients was higher than the virginal recipients (P＞0.05).
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