Research Of Microtuber Rapid-propagation In Field Of Virus-free Potato

Virus-free potato production cost is relatively high in the establishment. Thepurpose of all the way of Virus-free potato production is to produce Minipotato, Ittake many process, long time, infected by virus and soon on. It is of benefit to lowerthe production cost, produce Virus-free potato which is fit for field, especiallyappropriate microtuber. In order to understand the effects of main factors ofsurvival Percent,morphology characteristics,yield and quality on the productionof transplanting Virus-free potato in the field., and choose the best planting whichis fit for Ya an and can be used to set up planting mode for Virus-free microtuberproduction (especial eligible microtuber). Through orthogonal tests with 5 factorsand different levels L₁₈ (6¹Ã—3⁴),the effect of it on transplanting Virus-free potatoin the field were studied. The results indicated that:1. The survival percent of the transplanting Virus-free potato was not high, the rangewas from 49.3ï¼…to 74.3ï¼…,it may be relative to environment. The best factor wastransplanting density for the effect of survival Percent, it has reached to verysignificant level; the second was plant incretion which has reached to significantlevel. The best level for plant incretion was IBA, the worst was IAA, there wassignificant difference, but between IBA and NAA, there was no significant difference.The best level for density was E2(75 palnt/m²),the worst was E1(25 palnt/m²),therewas significant difference, but between E2 and E3(50 palnt/m²), there was nosignificant difference2. The two factors-breed and nitrogenous fertilizer proportion have significanteffect on above growth of transplanting plant. For plant height, breed has reachedto very significant level, second was nitrogenous fertilizer and density, the twofactors reached significant level, but incretion do not reach significant level. ForPlant height, there was significant difference at different breed, the best wasDaXiYang; there was very significant difference at different nitrogenousfertilizer, the best was A3;E2 was best at different density. The same to Plant height,breed and nitrogenous fertilizer were main effect factor for stem width, leaf area and photosynthesis efficiency of leaves, but density was not. Breed have more effecton stem width and leaf area than nitrogenous fertilizer, breed was to very significantlevel, nitrogenous fertilizer was to significant level, TaiWanHong was the best forstem width and leaf area. For stem width, the breed gradation was: B2(TaiWanHong)＞B1(GaoYuan7)＞B3(DaXiYang);A3 was the best in different nitrogenousfertilizer. For leaf area, the breed gradation was B2(TaiWanHong)＞B3(DaXiYang)＞B1(GaoYuan7);A4 was the best. For photosynthesis efficiency of leaves, breed andnitrogenous fertilizer reached to significant level. The result of significantdifference at level test showed that:the best breed was TaiWanHong, nitrogenousfertilizer was A4.It was same to leaf area.3. It was different from usually plant for Under Growth of transplanting plant,especially root system forepart. The result of study of Under Growth showed that themain effect factor was incretion and concentration of incretion for root number andlength, incretion reached to very significant level, concentration was significantlevel. The best incretion was C3(IBA), concentration was D2(150mg/L)For root volume,the first effect factor was breed and concentration, they all reached to verysignificant level, the second was nitrogenous fertilizer, all reached to significantlevel. The result of test of different significant level showed that:the best breedwas B3, nitrogenous fertilizer was A3, incretion was C3, concentration was D2. Thestolen number of transplanting plant was lower,the most was 0.9 per transplantingplant, the least was 0.3 in early. In midway, the number was less than 1.0, generally,the range of number was 0.0-2.0, the same to microtuber production. The number of tuberwas lower, the most was 1.1,least was 0.1.4. The different combination had different effect on the number and weight of tuberper transplanting plant. Breed reached to very significant level, the density wasto significant level for number, but not for weight. The best breed was TaiWanHong, thedensity was E2 (75 palnt/m²),the worst was E1 (25 plant/m²),E3 (50 plant/m²)was second level. It was different from usually plant about the grade of tuber, thebig tuber proportion was very lower, the highest was 10.0ï¼…,some combinations had noone;the second was middling tuber, the highest was 38.5ï¼…;the highest proportion was small tuber, average proportion was above 50ï¼…,the highest reached to 83.3ï¼….Breedand nitrogenous fertilizer reached to very significant level for big tuberproportion,the best was TaiWangHong and A4. But for other, all factors had nosignificant effect.5. The different combination had different effect on yield, the highest was480.27g, lowest was 97.13g, the different was distinctness. Breed and density were twomain effect factor, the all reached very significant effect;nitrogenous fertilizerwas significant effect. The best was TaiWanHong, average was 345.87g, second wasGaoVuan7, 292.04g, the worst was DaXiYang, 190.95g. The best density was E2,nitrogenous fertilizer was A3. The best combination For yield was: A3 (15g/m²)+B2 (TaiWanHong) +C3 (IBA) +D2 (150mg/L) +E2 (75 plant/m²).nitrogenous fertilizerhad significant effect on potato quality, at the A3, the content of starch anddrymatter was higher than other levels, drymatter average was 19.96ï¼…, starch was15.85ï¼…;but the highest content of reducing sugar was at A4,0.053ï¼…,A6 waslowest, 0.034ï¼….Breed had no significant effect on the content of starch anddrymatter, but on reducing sugar, it was very significant effect factor. In 3breeds, GaoYuan7 was the highest, thelowest was DaXiYang.