Effect Of Vaccine Antigen Of FMD And The Component In Chinese Traditional Medicine On Micro-Vasomotion

To empolder a new kind of adjuvar of foot and mouth disease Vaccine from Chinese traditional medicine, and provide a theoretical basis for curing foot and mouth disease, we studied microvascular vasomotion on cells. The experiment was designed to culture the myocardial membrane microvascular cells(MMMVECs) from rat in vitro, we studied the effects of FMD Vaccine antigen on the excretion of Nitrous Oxide(NO) and Endothelin-1 (ET-1) of the MMMVECs, and the effects of the tetramethylpyrazine(TMP),extract of ginkgo biloba 761(EGb761) and anisodamine(654-2) added FMD Vaccine antigen on the excretion of factors of the MMMVECs. The result shows that: (1)The MMMVECs from rat were successfully cultivated and the system of MMMVECs was established. (2)The excretion of NO of the MMMVECs was increased significantly compared with the control by adding 0.1ul/ml FMD vaccine antigen to the culture medium(P＜0.01). This result indicated that the MMMVECs could be damaged directly by the FMD vaccine antigen. The excretion of ET-1 of the MMMVECs showed no significant difference compared with the control. (3)TMP(20ug/ml),EGb761(50ug/ml) and 654-2(20ug/ml) respectively mixed with FMD vaccine antigen(0.1ul/ml) were added to the culture medium, the excretion of NO of the MMMVECs was decreased significantly compared with the group of FMD vaccine antigen(P＜0.01). and it was steel up-regulated compared with the control. EGb761(50ug/ml) and 654-2(20ug/ml) respectively mixed with FMD vaccine antigen (0.1ul/ml) were added to the culture medium, The excretion of ET-1 of the MMMVECs was increased significantly compared with the control and the group of FMD vaccine antigen. This result indicated that the mixture of EGb761 and FMD vaccine antigen, and of 654-2 and FMD vaccine antigen could increase microvascular vasomotion extent.