| The system of Intestinal Mucous Membrane Microvascular Cells (IMECs) and the impairment models of the cells caused by the Lipopolysaccharide (LPS) and Heat-labile enterotoxin (LT) of E. coli were established to explores the effects of berberlne on the secretion functions of IMECs in order to provide technical and theoretical basis for the prevention and treatment of the E. coli disease with Chinese herbs. The experimental results are as follows:1.With the method of pasting tissue masses on the buttress, the IMECs be successfully cultivated and the system of IMECs be established.2.The LT carries out its effects through affecting the morphology and secreting functions of the IMECs, which differs from the generally accepted view that LT acts on the intestinal mucous membrane epithelium cells specifically.3.The morphological changes of IMECs with LPS are that the length/width ratio of a single cell and the space among cells are increased, and which of the IMECs with LT is that the cells become big with an irregular structure. These changes are related to the dose of these two toxins (LPS and LT).4. The levels of NO and ET secreted by IMECs with LPS are increased with different degrees. The ET level is 18.4 times as that as normal, but the NO is only 2.1 times as normal, which suggest that the impairment of LPS to IMECs is the result of increased secretion of ET, causing the contract of the IMECs.5. The level of NO secreted by IMECs increased with that of ET decreased at 6 hour of the LT stimulation. This indicates that LT could damage the IMECs by stimulating the secretion of NO to cause the extension of IMECs.6.A dose of 1 ug/ml of LPS is recommended for establishing the impairment model of IMECs, which will last for 9 hours. However, 50 ug/ml is an advisable dose for LT with a effective time lasting for 6 hours.7. Berberine can stimulate the secretion of NO, which keeps the IMECs in a state of extension. |
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