The Effect Of Hypothalamic-Pituitary-Adrenal-immune Organ In Rats By Additive Of Gamma Germ

Objective: This paper study the effect of Nervous-Endocrine-Immune System in rats by Additive of Gamma Germ. Method:①72 male rats were divided into 4 groups. Pab04 group was fed diet with additive of B. subtilis Pab04. PAS38 group was fed diet with additive of B. cereus PAS38. Antibiotics group was fed with Oxyteracycline. Control group was feed the basic diet. Immunohistochemistry SABC was used to measure ACTH of mouse hypothalamus, pituitarium, adrenal, splenic, thymus at the day of 20, 40, 60.②Immunohistochemistry SABC was used to measure CD4, CD8 of mouse splenic, thymus at the day of 20, 40, 60.③96 male rats were divided into 4 groups. MTT was used to measure the transformation of mouse splenic lymphocyte at the day of 14, 21, 28, 35, 42, 49, 56, 63.Conclusions:①The results indicated that Pab04 or PAS38 both have stably depressant effect on ACTH in mouse hypothalamus, pituitarium, adrenal, splenic, thymus, as well as reduce the production and secrete of ACTH. Microecology praeparation can reduced the contents of ACTH of tissue and enhance immunity.②Microecology praeparation has utility effective on rats CD4, CD8. CD4/CD8 has the tendency to normal value. The changes of splenic CD4/CD8 in Pab04 group mice are 1.74±0.35, 2.37±0.23, 2.28±0.65 during 60 days; PAS38 group mice are 2.17±0.49, 2.63±0.41, 2.55±0.56; antibiotics group mice are 1.74±0.22, 4.11±1.83, 0.81±0.26; control group mice are 1.26 ±0.31, 0.94±0.42, 2.26±0.56. Antibiotics and control group make big changes of CD4/CD8 in splenic during 60deys. It is disadvantaged for bodies to homeostasis.③The results indicated that Pab04 or PAS38 preparation improved the transformation of rat splenic lymphocyte respectively. This results were observe during th whole experiment that microecology preparation can improve the immune of rats.