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The Morphological Observation Of Floral Organs And Gene Mapping Of A Non-palea Mutant In Rice

Posted on:2008-04-18Degree:MasterType:Thesis
Country:ChinaCandidate:H J PengFull Text:PDF
GTID:2143360218453889Subject:Seed science and engineering
Abstract/Summary:
Rice (Oryza sativa L.) is a model plant for study of the molecular developmentbiology in monocots. So the studies on rice inflorescence and on floral organs havebecome one of the most interesting fields in rice molecular research. In this study, wehave the investigation of the anatomical structure of floral organs, genetic analysis ofmutant traits and molecular tagging of genes of the non-palea mutant. The mainresults are summarized as follows:1. Non-palea mutant floral organs compared with the normal rice floral organslack palea and generate two addition leaf-like organs, number of carpels is changed insome floral organs. In additions, there are floral organs with two lammers.2. We made a cross between the mutant and the wild-type 99P258. All the F1plants displayed wild-type phenotype, while the F2 progenies segregated in a ratio of 3normal: 1 non-palea. Results of genetic analysis indicated that the traits of npa-2mutant is controlled by a single recessive gene.3. The F2 population, derived from non-palea mutant×99P258, was used toscreen molecular markers linked with non-palea gene. The gene was mapped betweentwo simple sequence repeat markers, RM4784 and RM19367 on chromosome 6,wihdistances of 2.9cM and 5.0cM, respectively. On account of the resemble gene ofnpa-1, so this gene is designated as npa-2.
Keywords/Search Tags:rice, the non-palea mutant, MADS-box gene, genetic analysis, gene mapping
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