Gene Mapping Of Dicer-Like5 Mutant Gene In Rice

In this study, varieties rice 001 were chemically induced by ethyl methane sulphonate(EMS),in its offspring,a DCL5 mutant plant appeared. The plants showed the lemma being partially or completely degenerated to the awn,similar to DCL4,but with DCL4 is not the same DCL5 lemma curled up into a paper clip. Each round of degradation of floral organ development,Can’t normal strong,pistil dysplasia,this phenomenon cannot be fully explained by the ABC model of flower organ development,nor by the DC L4 si RN A to explain.In order to determine the genetic law of of the mutant trait,the DC L5 mutant did reciprocal hybridization,respectively with Da lixiang.In the F1 generation,found that the offspring’s all plants showed the wild-type traits,the result show the mutant trait is controlled by recessive gene;The separation of the traits appeared in the F2 generation,and the separation ratio of wild-type plants and mutant plants meets with the genetic law of the Mendel’s 3:1 single recessive gene by the chi-square test,so it is showed that the mutant trait is controlled by a single recessive gene.DCL5 gene mapping were constructed from a cross between the DC L5 mutations heterozygotes and large incense.SSR(simple sequence repeats)maekers were used for molecular mapping.At first,the polymorphisms between the DC L5 mutations heterozygotes and large incense were examined with 400 pairs of SSR primers of RM series,of which 22 pairs exhibited polymorphism.Then these 22 primer-pairs,10 mutant plants,and 10 normal plants from F2 population were used for linkage analysis between markers and target genes,and the polymorphic linkage markers were used to survey a total of 144 F2 plants derived from a cross between the DC L5 mutations heterozygotes and large incense.The DCL5 gene was preliminarily mapped the molecular markers RM6266 in the short arm of chromosome 1,with a respective distance of 17.7c M.1999 mutant individuals identified from a big population were used for fine mapping. SSR molecular marker RM15316, RM5488 and RM6266 is located in the same side, with distances of 0.03 c M, 0.03 c M. SSR molecular marker RM15280 on the other side, the target gene and wihe distance of 0.2 c M. RM15293 may for the intragenic markers, genetic distance is 0.0 c M.